NFX1

General Information

Gene Name : NFX1
Gene ID : ENSG00000086102.18
Essential Gene : No

Functions

Novel RBP

Localization(RBPImage: NFX1)

Nuclei
Nucleoplasm
Mutually Exclusive from Nucleolus
Cytoplasm
Compartment/Organelle
Unknown Cyto Foci
Cytosolic
Cell Cortex

Domains (AlphaFold: NFX1)

No checked domain (RRM, ZNF, KH, Helicase, Nuclease, dRBM or PUM_HD)

Antibody Validation

View validation images for all NFX1 antibodies

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	NFX1	ENSG00000086102.18	29.01	30.23	29.62	ENCSR000AEF
HepG2	NFX1	ENSG00000086102.18	19.71	24.45	22.08	ENCSR985KAT
K562	NFX1	ENSG00000086102.18	23.66	25.89	24.77	ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
knockdown followed by RNA-seq	NFX1-BGKLV13-K562	ENCSR007XKL	NFX1	K562	Released		ENCSR815CVQ	shRNA	KD% = ,
knockdown followed by RNA-seq	NFX1-BGHcLV10-HepG2	ENCSR548ORV	NFX1	HepG2	Released		ENCSR775ZWO	CRISPR	KD% = 65.9,60.7

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
NFX1	567	K562	ENCSR276LGL	ENCSR821ESG	ENCAB841CSS	A302-914A	1	Revoked	ENCODE3

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
NFX1	ENCAB841CSS	A302-914A	1			Released, ENCODE3	BGHcLV10-29, Released, ENCODE4
NFX1		A302-916A	1				BGHcLV10-29, Released, ENCODE4	BGKcLV44-35, Submitted, ENCORE2

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
NFX1	K562	shRNA	ENCSR007XKL	ENCSR815CVQ	NFX1_BGKLV13_25,NFX1_BGKLV13_26	NT_BGKLV13_1,NT_BGKLV13_2	,	Released	ENCODE3
NFX1	K562	shRNA			BGKLV28-29,BGKLV28-30	BGKLV28-1,BGKLV28-2	9.6,39.9	NotSatisfied	ENCODE3
NFX1	HepG2	shRNA			BGHLV23-23,BGHLV23-24	BGHLV23-1,BGHLV23-2	redo transfection,redo transfection	NotSatisfied	ENCODE3
NFX1	HepG2	shRNA			BGHLV26-25,BGHLV26-26	BGHLV26-1,BGHLV26-2	25.0,12.7	NotSatisfied	ENCODE3
NFX1	K562	CRISPR			BGKcLV02-13,BGKcLV02-14	BGKcLV02-1,BGKcLV02-2	0.0,0.0	NotSatisfied	ENCODE3
NFX1	K562	CRISPR			BGKcLV08-41,BGKcLV08-42	BGKcLV08-1,BGKcLV08-2	14.5,1.3	NotSatisfied	ENCODE4
NFX1	K562	CRISPR			NFX1-BGKcLV44-35,NFX1-BGKcLV44-36	NT-BGKcLV44-1,NT-BGKcLV44-2	61.6,61.9	Submitting	ENCORE2
NFX1	HepG2	CRISPR			BGHcLV03-11,BGHcLV03-12	BGHcLV03-1,BGHcLV03-2	0.0,0.0	NotSatisfied	ENCODE3
NFX1	HepG2	CRISPR	ENCSR548ORV	ENCSR775ZWO	NFX1-BGHcLV10-29,NFX1-BGHcLV10-30	NT-BGHcLV10-1,NT-BGHcLV10-2	65.9,60.7	Released	ENCODE4
NFX1	HepG2	CRISPR			BGHcLV10-31,BGHcLV10-32	BGHcLV10-1,BGHcLV10-2		NotSatisfied	ENCODE4
NFX1	HepG2	CRISPR			BGHcLV45-45,BGHcLV45-46	BGHcLV45-1,BGHcLV45-2		NotSatisfied	ENCORE2

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

NFX1: Click to RBP Image Database

Nuclei

Nucleoplasm

Mutually Exclusive from Nucleolus

Cytoplasm

Compartment/Organelle

Unknown Cyto Foci

Cytosolic

Cell Cortex

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
NFX1	Product_ID: A302-914A Lot_ID: 1 Source: Bethyl Labs Target Name: NFX1-human
NFX1	Product_ID: A302-916A Lot_ID: 1 Source: Bethyl Labs Target Name: NFX1-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for NFX1 is not avaliable on ENCORE database.

Proteins bound to RNA

NFX1 RNA is bound by 17 proteins.

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eCLIP assay for NFX1 is not avaliable on ENCORE database.

17 proteins are bound to NFX1 RNA:

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Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr9	33294827	33294993	+	CDS	4.13775	17.19016	HepG2	BCLAF1
chr9	33301361	33301420	+	CDS,intron-Proximal	4.21150	10.57092	HepG2	BCLAF1
chr9	33313778	33313831	+	CDS,intron-Proximal	3.74658	9.70941	HepG2	PRPF8
chr9	33295351	33295420	+	CDS	4.54908	9.47159	HepG2	BCLAF1
chr9	33366063	33366153	+	intron-Distal,intron-Proximal	5.75830	8.60492	HepG2	PCBP2
chr9	33294721	33294827	+	CDS	3.41182	8.50351	HepG2	BCLAF1
chr9	33303222	33303265	+	CDS	4.45011	8.41475	HepG2	BCLAF1
chr9	33318785	33318828	+	CDS	5.33762	8.40988	HepG2	BCLAF1
chr9	33293702	33293775	+	intron-Distal	5.79894	8.38577	HepG2	PRPF8
chr9	33303183	33303262	+	CDS,intron-Proximal	3.81432	8.12130	HepG2	PPIG
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 44 entries

eCLIP assay for NFX1 is not avaliable on ENCORE database.

Diffrential Expression

49 differentially expressed genes after Knockdown/Knockout NFX1 protein.

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50 RBPs can affect the levels of NFX1.

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49 differentially expressed genes after Knockdown/Knockout NFX1 protein :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
FAM203B	ENSG00000230567.3	71.16080	0.00000	99999.99990	20.85580	16.49110	K562	NFX1
PRG2	ENSG00000186652.5	960.13270	247.25170	1.95720	19.48850	15.42490	K562	NFX1
ZNF816-ZNF321P	ENSG00000213801.4	20.36700	170.66130	-3.06680	18.98020	15.09270	K562	NFX1
IFITM1	ENSG00000185885.11	2156.73830	7133.04700	-1.72570	15.48460	11.72200	K562	NFX1
EGR1	ENSG00000120738.7	687.54690	1930.89790	-1.48970	13.32480	9.65920	K562	NFX1
MT2A	ENSG00000125148.6	388.96130	124.31870	1.64550	11.20430	7.61780	K562	NFX1
RNF167	ENSG00000108523.11	342.82440	829.92340	-1.27550	9.10480	5.58530	K562	NFX1
FAM138F	ENSG00000233630.4	105.02330	23.10660	2.18420	8.82450	5.36290	K562	NFX1
COL1A1	ENSG00000108821.9	164.79350	47.91630	1.78200	8.61730	5.20690	K562	NFX1
APOE	ENSG00000130203.5	1044.90640	469.49080	1.15410	7.90000	4.53540	K562	NFX1
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 49 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

50 RBPs can affect the gene expression of NFX1 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
NFX1	ENSG00000086102.14	1622.35890	922.73240	0.81400	16.38840	15.02340	K562	FXR1
NFX1	ENSG00000086102.14	2011.97570	1428.77210	0.49370	10.30930	9.44040	HepG2	SSB
NFX1	ENSG00000086102.14	864.69650	1296.21560	-0.58400	10.04650	9.05280	K562	AGGF1
NFX1	ENSG00000086102.14	3047.19750	2265.07430	0.42780	8.55570	7.49060	K562	DDX47
NFX1	ENSG00000086102.14	1415.65810	1005.13250	0.49400	6.84470	5.60440	K562	EIF3G
NFX1	ENSG00000086102.14	913.02240	1337.72120	-0.55110	5.49900	4.76550	K562	PUS1
NFX1	ENSG00000086102.14	847.79140	1257.51820	-0.56880	5.34430	4.49900	HepG2	HNRNPLL
NFX1	ENSG00000086102.14	1176.90100	887.98020	0.40630	5.03630	4.17170	HepG2	DKC1
NFX1	ENSG00000086102.14	937.73650	1299.58500	-0.47080	4.55610	3.81290	HepG2	PUF60
NFX1	ENSG00000086102.14	1319.39280	1645.57690	-0.31870	4.29040	3.70610	HepG2	SRFBP1
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 53 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for NFX1 is not available on ENCORE database.

2 RBPs can affect the splicing of NFX1 :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP	Bound by RBP
SE	NFX1	ENSG00000086102.14	chr9	+	33291418	33291576			33290508	33290595	33294417	33295425	0.13800	0.00005	0.00360	included	HepG2	MATR3
A3SS	NFX1	ENSG00000086102.14	chr9	+	33311084	33311175			33311103	33311175	33307191	33307297	0.06800	0.00030	0.00237	longer	K562	AQR

Showing 1 to 2 of 2 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

NFX1

General Information

Functions

Localization(RBPImage: NFX1)

Domains (AlphaFold: NFX1)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for NFX1 is not avaliable on ENCORE database.

17 proteins are bound to NFX1 RNA:

eCLIP assay for NFX1 is not avaliable on ENCORE database.

Diffrential Expression

49 differentially expressed genes after Knockdown/Knockout NFX1 protein :

50 RBPs can affect the gene expression of NFX1 :

The Knockdown/knockout RNA-Seq assay for NFX1 is not available on ENCORE database.

2 RBPs can affect the splicing of NFX1 :

The Knockdown/knockout RNA-Seq assay for NFX1 is not avaliable on ENCORE database.