IFIT2

General Information

Gene Name       : IFIT2
Gene ID             : ENSG00000119922.9
Essential Gene : No

Functions

Localization

Domains (AlphaFold: IFIT2)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878IFIT2ENSG00000119922.99.466.527.99ENCSR000AEF
HepG2IFIT2ENSG00000119922.90.040.090.07ENCSR985KAT
K562IFIT2ENSG00000119922.90.060.020.04ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq IFIT2-BGKcLV17-K562ENCSR671OGOIFIT2K562ReleasedENCSR516TLX   CRISPRKD% = 49.6,50.6
knockdown followed by RNA-seq IFIT2-BGHcLV18-HepG2ENCSR144HCMIFIT2HepG2ReleasedENCSR670KAS   CRISPRKD% = ,






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
IFIT2ENCAB688YERPA5-28800TK2671326AReleased, ENCODE3BGKcLV17-31, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
IFIT2K562CRISPRENCSR671OGOENCSR516TLXIFIT2-BGKcLV17-31,IFIT2-BGKcLV17-32NT-BGKcLV17-1,NT-BGKcLV17-249.6,50.6ReleasedENCODE4
IFIT2HepG2CRISPRENCSR144HCMENCSR670KASIFIT2-BGHcLV18-27,IFIT2-BGHcLV18-28NT-BGHcLV18-1,NT-BGHcLV18-2,ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
IFIT2Product_ID: PA5-28800
Lot_ID: TK2671326A
Source: Thermo Fisher
Target Name: IFIT2-human		 ThermoFisher_PA5-28800_TK2671326A_IFIT2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the IFIT2 specific antibody, PA5-28800. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the IFIT2-specific antibody, PA5-28800. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 IFIT2-K562-CRISPR-MA5-28800.png<br>Caption: Western blot following CRISPR against IFIT2 in K562 whole cell lysate using IFIT2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against IFIT2. IFIT2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for IFIT2 is not avaliable on ENCORE database.



Proteins bound to RNA

  • NO known proteins bind on IFIT2 RNA with finished eCLIP experiments on ENCORE database.





eCLIP assay for IFIT2 is not avaliable on ENCORE database.






NO known proteins are bound to IFIT2 RNA with finished eCLIP experiments on ENCORE database.





eCLIP assay for IFIT2 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for IFIT2 is not available on ENCORE database.

  • 26 RBPs can affect the levels of IFIT2.  








The Knockdown/knockout RNA-Seq assay for IFIT2 is not available on ENCORE database.





26 RBPs can affect the gene expression of IFIT2 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
IFIT2ENSG00000119922.735.174009.854201.835602.905502.10310HepG2ASCC1
IFIT2ENSG00000119922.751.541208.394002.618206.081204.60940HepG2CCAR1
IFIT2ENSG00000119922.739.863309.363102.089903.777202.88350HepG2CIRBP
IFIT2ENSG00000119922.764.0746011.405802.489906.068905.17680HepG2DDX3X
IFIT2ENSG00000119922.735.157102.460103.836904.624903.65200HepG2DDX55
IFIT2ENSG00000119922.724.425103.644702.744403.553602.80160HepG2DKC1
IFIT2ENSG00000119922.736.903209.418401.970103.232102.59330HepG2EIF2S1
IFIT2ENSG00000119922.727.156804.711602.526903.265502.57140HepG2HNRNPA1
IFIT2ENSG00000119922.724.859804.660702.415103.684802.86690HepG2HNRNPF
IFIT2ENSG00000119922.732.614404.589602.829005.411704.43250HepG2IGF2BP1
IFIT2ENSG00000119922.747.559609.900502.264104.999304.11600HepG2IGF2BP2
IFIT2ENSG00000119922.718.578301.886803.299502.437401.67730HepG2PRPF8
IFIT2ENSG00000119922.736.546407.098102.364103.996203.29470HepG2PUF60
IFIT2ENSG00000119922.730.882105.019002.621204.334203.50850HepG2RPLP0
IFIT2ENSG00000119922.719.907802.889402.784402.921702.10070HepG2RPS3A
IFIT2ENSG00000119922.757.709504.578603.6557012.5105011.21520HepG2SRSF7
IFIT2ENSG00000119922.727.6170011.196501.302401.839601.39990HepG2SSB
IFIT2ENSG00000119922.722.691506.117101.891101.924001.38920HepG2U2AF1
IFIT2ENSG00000119922.737.162907.134002.381004.733603.39940HepG2UTP18
IFIT2ENSG00000119922.713.241001.784102.891602.070801.54870K562AQR
IFIT2ENSG00000119922.717.176102.774402.630002.240501.30410K562CNOT7
IFIT2ENSG00000119922.712.056001.632002.884901.993201.36530K562EIF2S2
IFIT2ENSG00000119922.714.645902.599402.494202.023301.49010K562FXR1
IFIT2ENSG00000119922.717.244302.865202.589302.313101.58670K562GPKOW
IFIT2ENSG00000119922.722.684601.031104.459404.839304.07070K562HNRNPC
IFIT2ENSG00000119922.741.227405.914002.801305.612804.45380K562PUF60
IFIT2ENSG00000119922.712.943202.007602.688502.205701.44580K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for IFIT2 is not available on ENCORE database.






No RBPs affect the alternative splcing events of IFIT2 on ENCORE database.





The Knockdown/knockout RNA-Seq assay for IFIT2 is not avaliable on ENCORE database.