E4F1

General Information

Gene Name : E4F1
Gene ID : ENSG00000167967.15
Essential Gene : Yes

Functions

Not Available

Localization

Not Available

Domains (AlphaFold: E4F1)

Not Available

Antibody Validation

Thermo Fisher: PA5-67167

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	E4F1	ENSG00000167967.15	0.20	1.55	0.88	ENCSR000AEF
HepG2	E4F1	ENSG00000167967.15	18.19	1.80	10.00	ENCSR985KAT
K562	E4F1	ENSG00000167967.15	16.75	3.77	10.26	ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
knockdown followed by RNA-seq	E4F1-BGKcLV17-K562	ENCSR916PCG	E4F1	K562	Released		ENCSR516TLX	CRISPR	KD% = 65.9,74.7
knockdown followed by RNA-seq	E4F1-BGHcLV18-HepG2	ENCSR308EYT	E4F1	HepG2	Released		ENCSR670KAS	CRISPR	KD% = 97.4,97.9

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
E4F1	ENCAB559HVW	PA5-67167	UC2739267A			NotSatisfied, ENCODE4	BGHcLV18-15, OnHold, ENCODE4	BGKcLV17-15, Released, ENCODE4

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project	Note
E4F1	K562	CRISPR	ENCSR916PCG	ENCSR516TLX	E4F1-BGKcLV17-15,E4F1-BGKcLV17-16	NT-BGKcLV17-1,NT-BGKcLV17-2	65.9,74.7	Released	ENCODE4
E4F1	HepG2	CRISPR	ENCSR308EYT	ENCSR670KAS	E4F1-BGHcLV18-15,E4F1-BGHcLV18-16	NT-BGHcLV18-1,NT-BGHcLV18-2	97.4,97.9	Released	ENCODE4

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

No Localization Information available

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
E4F1	Product_ID: PA5-67167 Lot_ID: UC2739267A Source: Thermo Fisher Target Name: E4F1-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for E4F1 is not avaliable on ENCORE database.

Proteins bound to RNA

E4F1 RNA is bound by 38 proteins.

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eCLIP assay for E4F1 is not avaliable on ENCORE database.

38 proteins are bound to E4F1 RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr16	2285621	2285728	+	3'-UTR	6.25105	35.59442	K562	PUM2
chr16	2283183	2283229	+	CDS,intron-Proximal	5.17656	35.11188	HepG2	PRPF8
chr16	2283217	2283240	+	intron-Proximal	5.75644	29.91119	K562	AQR
chr16	2283369	2283448	+	CDS,intron-Proximal	5.23016	28.69094	K562	SF3B4
chr16	2282408	2282472	+	3'-UTR,CDS,intron-Proximal	4.24949	27.75835	K562	SF3B4
chr16	2283191	2283217	+	intron-Proximal	5.63193	27.49803	K562	AQR
chr16	2283410	2283482	+	CDS,intron-Proximal	4.81125	25.11153	K562	AQR
chr16	2283183	2283230	+	CDS,intron-Proximal	6.57284	23.48009	HepG2	AQR
chr16	2278285	2278379	+	CDS,intron-Proximal	4.79224	22.53414	K562	SF3B4
chr16	2278548	2278619	+	intron-Proximal	4.22316	22.02483	K562	AQR
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 139 entries

eCLIP assay for E4F1 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout assay for E4F1 is not available on ENCORE database.
112 RBPs can affect the levels of E4F1.

CanvasJS.com

The Knockdown/knockout RNA-Seq assay for E4F1 is not available on ENCORE database.

112 RBPs can affect the gene expression of E4F1 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
E4F1	ENSG00000167967.11	2339.65190	855.65790	1.45110	32.59290	30.70170	K562	PUS1
E4F1	ENSG00000167967.11	595.54690	160.37420	1.89270	27.41450	25.50290	HepG2	MAGOH
E4F1	ENSG00000167967.11	1144.21410	382.08730	1.58230	28.74120	25.35100	K562	EFTUD2
E4F1	ENSG00000167967.11	477.05340	167.08630	1.51350	26.17910	24.52890	HepG2	SRSF5
E4F1	ENSG00000167967.11	534.31910	150.70030	1.82590	25.47440	23.57610	HepG2	HNRNPK
E4F1	ENSG00000167967.11	552.96710	197.49260	1.48530	24.67730	23.38190	HepG2	NELFE
E4F1	ENSG00000167967.11	706.01440	276.78770	1.35080	24.65740	22.76040	K562	PTBP1
E4F1	ENSG00000167967.11	1094.05260	347.98000	1.65250	24.56950	22.51170	HepG2	SF3A3
E4F1	ENSG00000167967.11	455.36370	179.63700	1.34180	23.34880	21.96810	HepG2	PUM1
E4F1	ENSG00000167967.11	1898.99800	833.94300	1.18710	21.03230	19.34370	K562	RBM15
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 132 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for E4F1 is not available on ENCORE database.

8 RBPs can affect the splicing of E4F1 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
RI	E4F1	ENSG00000167967.11	chr16	+	2279570	2282365	2279570	2279676	2282171	2282365	0.33600	0.00000	0.00001	included	K562	PUS1
RI	E4F1	ENSG00000167967.11	chr16	+	2279570	2282365	2279570	2279676	2282171	2282365	0.36500	0.00000	0.00000	included	K562	AQR
A5SS	E4F1	ENSG00000167967.11	chr16	+	2284859	2285002	2284859	2284972	2285081	2285144	-0.10500	0.00006	0.00170	shorter	K562	AQR
RI	E4F1	ENSG00000167967.11	chr16	+	2284701	2284950	2284701	2284782	2284859	2284950	0.05600	0.00041	0.04728	included	K562	PUM1
RI	E4F1	ENSG00000167967.11	chr16	+	2279570	2282365	2279570	2279676	2282171	2282365	-0.36900	0.00061	0.01723	excluded	K562	HNRNPC
A5SS	E4F1	ENSG00000167967.11	chr16	+	2284859	2285002	2284859	2284972	2285081	2285144	-0.11600	0.00074	0.06549	shorter	K562	PRPF8
RI	E4F1	ENSG00000167967.11	chr16	+	2279570	2282365	2279570	2279676	2282171	2282365	0.36100	0.00099	0.02057	included	HepG2	FUBP3
RI	E4F1	ENSG00000167967.11	chr16	+	2279570	2282365	2279570	2279676	2282171	2282365	0.22400	0.00607	0.05166	included	K562	AKAP8L
RI	E4F1	ENSG00000167967.11	chr16	+	2279570	2282365	2279570	2279676	2282171	2282365	0.45400	0.01024	0.06322	included	HepG2	SF3B4

Showing 1 to 9 of 9 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

E4F1

General Information

Functions

Localization

Domains (AlphaFold: E4F1)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for E4F1 is not avaliable on ENCORE database.

38 proteins are bound to E4F1 RNA:

eCLIP assay for E4F1 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout RNA-Seq assay for E4F1 is not available on ENCORE database.

112 RBPs can affect the gene expression of E4F1 :

The Knockdown/knockout RNA-Seq assay for E4F1 is not available on ENCORE database.

8 RBPs can affect the splicing of E4F1 :

The Knockdown/knockout RNA-Seq assay for E4F1 is not avaliable on ENCORE database.