| HepG2 | 
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using PTBP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PTBP1 antibody (lanes under 'PTBP1'). Method: immunoprecipitation Releated Sample: eCLIP:696 Status: Released Lab: Yeo Lab | 
Caption: Western blot following shRNA against PTBP1 in K562 and HepG2 whole cell lysate using PTBP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PTBP1. Lanes 5-7 follow the same pattern, but in HepG2. PTBP1 protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV11-35 Status: Released Lab: Graveley Lab |
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| K562 | 
Caption: IP-Western Blot analysis of K562 whole cell lysate using PTBP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PTBP1 antibody (lanes under 'PTBP1'). Method: immunoprecipitation Releated Sample: eCLIP:368 Status: Released Lab: Yeo Lab |
Caption: Western blot following shRNA against PTBP1 in K562 and HepG2 whole cell lysate using PTBP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PTBP1. Lanes 5-7 follow the same pattern, but in HepG2. PTBP1 protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV11-35 Status: Released Lab: Graveley Lab 
Caption: Western blot following CRISPR against PTBP1 in K562 whole cell lysate using PTBP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PTBP1. PTBP1 protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKcLV02-7 Status: Released Lab: Graveley Lab |
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