Antibodies of PTBP1


5 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
PTBP1Product_ID: RN011P
Lot_ID: 1
Source: MBLI
Target Name: PTBP1-human		 HepG2_MBL_RN011P_001_PTBP1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using PTBP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PTBP1 antibody (lanes under 'PTBP1').
 PTBP1_Secondary_Western.png<br>Caption: Western blot following shRNA against PTBP1 in K562 and HepG2 whole cell lysate using PTBP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PTBP1. Lanes 5-7 follow the same pattern, but in HepG2. PTBP1 protein appears as the green band, GAPDH serves as a control and appears in red.
 K562_MBLI_RN011P_001_PTBP1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using PTBP1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PTBP1 antibody (lanes under 'PTBP1').
 PTBP1_Secondary_Western.png<br>Caption: Western blot following shRNA against PTBP1 in K562 and HepG2 whole cell lysate using PTBP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against PTBP1. Lanes 5-7 follow the same pattern, but in HepG2. PTBP1 protein appears as the green band, GAPDH serves as a control and appears in red.
PTBP1-K562-CRISPR.png<br>Caption: Western blot following CRISPR against PTBP1 in K562 whole cell lysate using PTBP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PTBP1. PTBP1 protein appears as the green band, GAPDH serves as a control and appears in red.
PTBP1Product_ID: 32-4800
Lot_ID: TG268402
Source: Thermo Fisher
Target Name: PTBP1-human		 PTBP1-HEPG2.png<br>Caption: Western blot following shRNA against PTBP1 in HepG2 whole cell lysate using PTBP1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against PTBP1. PTBP1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 ThermoFisher_32-4800_TG268402_PTBP1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the PTBP1 specific antibody, 32-4800. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the PTBP1-specific antibody, 32-4800. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 PTBP1-K562-CRISPR-1.png<br>Caption: Western blot following CRISPR against PTBP1 in K562 whole cell lysate using PTBP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PTBP1. PTBP1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
PTBP1Product_ID: ARP40421_P050
Lot_ID: QC10301
Source: Aviva
Target Name: PTBP1-human		 PTBP1_aviva-1_WB_HeLa_Fu.png<br>Caption: Western blot analysis of lysates from HeLa cells using rabbit polyclonal to PTBP1. Expected size: ~57.2kDa
PTBP1Product_ID: 57246S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: PTBP1-human		 PTBP1-HEPG2-57246S.png<br>Caption: Western blot following shRNA against PTBP1 in HepG2 whole cell lysate using PTBP1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against PTBP1. PTBP1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_57246S_1_PTBP1.png<br>Caption: IP-WB analysis of 57246S whole cell lysate using the PTBP1 specific antibody, 57246S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the PTBP1-specific antibody, 57246S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
PTBP1Product_ID: 72669S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: PTBP1-human		 PTBP1-HEPG2-72669S.png<br>Caption: Western blot following shRNA against PTBP1 in HepG2 whole cell lysate using PTBP1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against PTBP1. PTBP1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_72669S_1_PTBP1.png<br>Caption: IP-WB analysis of 72669S whole cell lysate using the PTBP1 specific antibody, 72669S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the PTBP1-specific antibody, 72669S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.