CAST

General Information

Gene Name : CAST
Gene ID : ENSG00000153113.23
Essential Gene : No

Functions

Not Available

Localization

Not Available

Domains (AlphaFold: CAST)

Not Available

Antibody Validation

View validation images for all CAST antibodies

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	CAST	ENSG00000153113.23	87.35	95.39	91.37	ENCSR000AEF
HepG2	CAST	ENSG00000153113.23	40.50	57.66	49.08	ENCSR985KAT
K562	CAST	ENSG00000153113.23	83.54	91.62	87.58	ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
knockdown followed by RNA-seq	CAST-BGKcLV26-K562	ENCSR159HPD	CAST	K562	Released		ENCSR900GQL	CRISPR	KD% = 78.8,86.4

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	K562 Primary Validation	K562 Secondary Validation
CAST	ENCAB248JEG	A304-794A	1	Released, ENCODE4	BGKcLV26-59, Released, ENCODE4
CAST	ENCAB206XCH	A301-295A	1	Released, ENCODE4
CAST	ENCAB930NNF	A304-793A	1	Released, ENCODE4

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
CAST	K562	CRISPR	ENCSR159HPD	ENCSR900GQL	CAST-BGKcLV26-59,CAST-BGKcLV26-60	NT-BGKcLV26-1,NT-BGKcLV26-2	78.8,86.4	Released	ENCODE4
CAST	HepG2	CRISPR			BGHcLV27-41,BGHcLV27-42	BGHcLV27-1,BGHcLV27-2	0.0,0.0	NotSatisfied	ENCODE4
CAST	HepG2	CRISPR			BGHcLV49-5,BGHcLV49-6	BGHcLV49-1,BGHcLV49-2	54.7,50.4128	NotSatisfied	ENCORE2

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

No Localization Information available

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
CAST	Product_ID: A301-295A Lot_ID: 1 Source: Bethyl Labs Target Name: CAST-human
CAST	Product_ID: A304-793A Lot_ID: 1 Source: Bethyl Labs Target Name: CAST-human
CAST	Product_ID: A304-794A Lot_ID: 1 Source: Bethyl Labs Target Name: CAST-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for CAST is not avaliable on ENCORE database.

Proteins bound to RNA

CAST RNA is bound by 23 proteins.

CanvasJS.com

eCLIP assay for CAST is not avaliable on ENCORE database.

23 proteins are bound to CAST RNA:

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Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr5	96086288	96086378	+	CDS,intron-Proximal	4.77327	25.43760	K562	UCHL5
chr5	96086290	96086355	+	CDS,intron-Proximal	4.46841	21.13832	K562	ZNF622
chr5	96090360	96090446	+	CDS,intron-Proximal	3.68108	18.97774	K562	UCHL5
chr5	96084244	96084319	+	CDS,intron-Proximal	4.31398	17.96214	HepG2	BCLAF1
chr5	96084263	96084319	+	CDS,intron-Proximal	4.62958	16.75438	K562	UCHL5
chr5	96073574	96073636	+	CDS	3.45349	15.80944	HepG2	BCLAF1
chr5	96084220	96084319	+	CDS,intron-Proximal	3.05978	15.55524	K562	ZNF800
chr5	96097981	96098058	+	CDS	4.65935	13.88979	HepG2	GRWD1
chr5	96073569	96073652	+	CDS,intron-Proximal	4.75523	13.79295	HepG2	GRWD1
chr5	96073636	96073653	+	CDS,intron-Proximal	4.61798	13.76815	HepG2	BCLAF1
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 130 entries

eCLIP assay for CAST is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout assay for CAST is not available on ENCORE database.
98 RBPs can affect the levels of CAST.

CanvasJS.com

The Knockdown/knockout RNA-Seq assay for CAST is not available on ENCORE database.

98 RBPs can affect the gene expression of CAST :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
CAST	ENSG00000153113.19	4895.61140	2113.93530	1.21150	80.09750	78.00520	HepG2	ACO1
CAST	ENSG00000153113.19	1621.84450	3083.71060	-0.92700	38.15250	35.76400	K562	MTPAP
CAST	ENSG00000153113.19	5309.04800	3315.16080	0.67930	29.01150	27.83500	HepG2	SRFBP1
CAST	ENSG00000153113.19	1735.59870	3058.67920	-0.81750	27.17880	25.42310	HepG2	AUH
CAST	ENSG00000153113.19	1066.52250	2469.34170	-1.21120	22.04650	20.36800	HepG2	NCBP2
CAST	ENSG00000153113.19	4271.20790	2609.63240	0.71070	21.67780	20.22540	HepG2	CELF1
CAST	ENSG00000153113.19	1692.42620	2622.51470	-0.63190	17.12950	15.76040	HepG2	SRSF5
CAST	ENSG00000153113.19	1330.24810	2528.40920	-0.92650	17.14140	15.59840	HepG2	TARDBP
CAST	ENSG00000153113.19	3263.53320	2305.85840	0.50100	16.39450	15.34120	HepG2	ZNF622
CAST	ENSG00000153113.19	1354.22030	2341.28430	-0.78980	15.44740	13.92350	HepG2	RBM25
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 107 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for CAST is not available on ENCORE database.

31 RBPs can affect the splicing of CAST :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	CAST	ENSG00000153113.19	chr5	+	96062497	96062563	96058342	96058402	96063192	96063234	0.21200	0.00001	included	HepG2	G3BP1
SE	CAST	ENSG00000153113.19	chr5	+	96106234	96106306	96103610	96103703	96107347	96107406	-0.23600	0.00000	excluded	K562	SF3B4
SE	CAST	ENSG00000153113.19	chr5	+	96064856	96064913	96063192	96063234	96065315	96065429	-0.24500	0.00000	excluded	HepG2	U2AF1
SE	CAST	ENSG00000153113.19	chr5	+	96066483	96066564	96065315	96065429	96071875	96071944	-0.12400	0.00000	excluded	HepG2	U2AF1
SE	CAST	ENSG00000153113.19	chr5	+	96082045	96082129	96078358	96078460	96083048	96083096	-0.12500	0.00000	excluded	HepG2	U2AF2
SE	CAST	ENSG00000153113.19	chr5	+	96076969	96077062	96075741	96075822	96077197	96077284	-0.54100	0.00000	excluded	HepG2	U2AF2
SE	CAST	ENSG00000153113.19	chr5	+	96064856	96064913	96063192	96063234	96065315	96065429	-0.32800	0.00000	excluded	HepG2	U2AF2
SE	CAST	ENSG00000153113.19	chr5	+	96062497	96062563	96058342	96058402	96063192	96063234	0.13500	0.00041	included	HepG2	AKAP1
SE	CAST	ENSG00000153113.19	chr5	+	96064856	96064913	96063192	96063234	96065315	96065429	-0.17000	0.00000	excluded	K562	PUS1
SE	CAST	ENSG00000153113.19	chr5	+	96064856	96064913	96063192	96063234	96065315	96065429	-0.29200	0.00000	excluded	K562	U2AF1

Showing 1 to 10 of 83 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

CAST

General Information

Functions

Localization

Domains (AlphaFold: CAST)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for CAST is not avaliable on ENCORE database.

23 proteins are bound to CAST RNA:

eCLIP assay for CAST is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout RNA-Seq assay for CAST is not available on ENCORE database.

98 RBPs can affect the gene expression of CAST :

The Knockdown/knockout RNA-Seq assay for CAST is not available on ENCORE database.

31 RBPs can affect the splicing of CAST :

The Knockdown/knockout RNA-Seq assay for CAST is not avaliable on ENCORE database.