HSPA9      CRISPR in HepG2      Batch: BGHcLV47

Experiment Information (Status: Sequenced)
BGHcLV47-41BGHcLV47-42
idx00
TRCN#_or_BGC#BGC#0001148BGC#0001148
shRNA_or_gRNA_sequenceTGGGGATACCTTCTTAGGTGTGGGGATACCTTCTTAGGTG
PAMGGGGGG
NameHSPA9_82HSPA9_82
Sample_IDBGHcLV47-41BGHcLV47-42
transduction_Date1/28/251/28/25
daysD6D6
RBP_nameHSPA9HSPA9
qPCR_result44.643.6
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc30883951
WB_result71.482.9
Ave_WB
WB_DONE_date3/18/25
MW75kd
IP
antibody_Cat#3593Slot # 2
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID98289829




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
HSPA9Product_ID: 3593S
Lot_ID: 2
Source: Cell Signaling Technology
Target Name: HSPA9-human		 HSPA9-HEPG2-CRISPR-3593S.png<br>Caption: Western blot following CRISPR against HSPA9 in HepG2 whole cell lysate using HSPA9 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against HSPA9. HSPA9 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_3593S_2_HSPA9.png<br>Caption: IP-WB analysis of 3593S whole cell lysate using the HSPA9 specific antibody, 3593S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the HSPA9-specific antibody, 3593S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 HSPA9-K562-CRISPR-3593S.png<br>Caption: Western blot following CRISPR against HSPA9 in K562 whole cell lysate using HSPA9 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against HSPA9. HSPA9 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGHcLV47-41BGHcLV47-42
Sample_IDBGHcLV47-41BGHcLV47-42
Sample Name
Sample NameHSPA9-BGHcLV47-41HSPA9-BGHcLV47-42
RBPHSPA9HSPA9
Cell_LineHepG2HepG2
Exp UID
StatusSequencedSequenced
Status_date2025-05-142025-05-14
ProjectENCORE2ENCORE2
Note
ID1416214163




Library-Prep Information
BGHcLV47-41BGHcLV47-42
Sample #1516
Sample NameHSPA9-BGHcLV47-41HSPA9-BGHcLV47-42
Sample_Name_Alias
Index Well PositionG09H09
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-04-232025-04-23
Lib_IDLib-250423Lib-250423
Tecan_Location
Tecan
Tecan_date
Size_bp251259
Peak_Molarity38.0042.20
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-04-222025-04-22
Sample_IDBGHcLV47-41BGHcLV47-42
RBPHSPA9HSPA9
Batch_IDBGHcLV47BGHcLV47
WB_result71.40082.900
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47264727




Sequencing Information
BGHcLV47-41BGHcLV47-42
Sample_IDBGHcLV47-41BGHcLV47-42
Sample NameHSPA9-BGHcLV47-41HSPA9-BGHcLV47-42
Pool IDPool-250422Pool-250422
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID34293430




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database