HNRNPA1 antibody: sc-32301


Antibody Info
Product_IDsc-32301
Lot_IDI2413
Host_organismmouse
SourceSanta Cruz Biotech
Antibody_DCC_IDENCAB603WLW
Target Info
GeneNameHNRNPA1
Phosphate
Target_nameHNRNPA1-human
Antibody Info
Product_IDsc-32301
Lot_IDE1011
Host_organismmouse
SourceSanta Cruz Biotech
Antibody_DCC_IDENCAB000AXP
Target Info
GeneNameHNRNPA1
Phosphate
Target_nameHNRNPA1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 Hepg2_Santa%20Cruz_sc-32301_I2413_HNRNPA1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using HNRNPA1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using mouse normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using mouse monoclonal anti-HNRNPA1 antibody (lanes under 'HNRNPA1').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using HNRNPA1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using mouse normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using mouse monoclonal anti-HNRNPA1 antibody (lanes under 'HNRNPA1').

Method: immunoprecipitation

Releated Sample: eCLIP:283

Status: Released

Lab: Yeo Lab

 HNRNPA1_Secondary_Western.png<br>Caption: Western blot following shRNA against HNRNPA1 in K562 and HepG2 whole cell lysate using HNRNPA1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against HNRNPA1. Lanes 5-8 follow the same pattern, but in HepG2. HNRNPA1 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against HNRNPA1 in K562 and HepG2 whole cell lysate using HNRNPA1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against HNRNPA1. Lanes 5-8 follow the same pattern, but in HepG2. HNRNPA1 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV21-17

Status: Released

Lab: Graveley Lab

K562 K562_Santa%20Cruz_sc-32301_I2413_HNRNPA1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using HNRNPA1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using mouse normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using mouse monoclonal anti-HNRNPA1 antibody (lanes under 'HNRNPA1').

Caption: IP-Western Blot analysis of K562 whole cell lysate using HNRNPA1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using mouse normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using mouse monoclonal anti-HNRNPA1 antibody (lanes under 'HNRNPA1').

Method: immunoprecipitation

Releated Sample: eCLIP:247

Status: Released

Lab: Yeo Lab

 HNRNPA1_Secondary_Western.png<br>Caption: Western blot following shRNA against HNRNPA1 in K562 and HepG2 whole cell lysate using HNRNPA1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against HNRNPA1. Lanes 5-8 follow the same pattern, but in HepG2. HNRNPA1 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against HNRNPA1 in K562 and HepG2 whole cell lysate using HNRNPA1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against HNRNPA1. Lanes 5-8 follow the same pattern, but in HepG2. HNRNPA1 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV21-17

Status: Released

Lab: Graveley Lab

Cell linePrimary validationSecondary validation
HepG2 HNRNPA1_HepG2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using HNRNPA1 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-HNRNPA1 antibody (lanes under 'anti-HNRNPA1'). Asterisk indicates light chain of antibody.

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using HNRNPA1 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-HNRNPA1 antibody (lanes under 'anti-HNRNPA1'). Asterisk indicates light chain of antibody.

Method: immunoprecipitation

Releated Sample: eCLIP:283

Status: Released

Lab: Fu Lab

K562 hnRNPA1_santacruz_IP-WB_K562_Fu.png<br>Caption: Immunoprecipitation from HeLa whole cell lysate and analized by western blot analysis uisng rabbit polyclonal to HNRNPA1

Caption: Immunoprecipitation from HeLa whole cell lysate and analized by western blot analysis uisng rabbit polyclonal to HNRNPA1

Comments: An IgG control is required according to the ENCODE antibody characterization standards.

Method: immunoprecipitation

Releated Sample: eCLIP:247

Status: NotSatisfied

Lab: Fu Lab