NONO antibody: sc-166702


Antibody Info
Product_IDsc-166702
Lot_IDC1810
Host_organismmouse
SourceSanta Cruz Biotech
Antibody_DCC_IDENCAB097MPS
Target Info
GeneNameNONO
Phosphate
Target_nameNONO-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 NONO_HepG2_sc.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using NONO specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-NONO antibody (lanes under 'anti-NONO'). Asterisk indicates heavy and light chains of antibody.

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using NONO specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-NONO antibody (lanes under 'anti-NONO'). Asterisk indicates heavy and light chains of antibody.

Method: immunoprecipitation

Releated Sample: eCLIP:4084

Status: Released

Lab: Fu Lab

NONO-HEPG2-FU%27S.png<br>Caption: Western blot following shRNA against NONO in HepG2 whole cell lysate using NONO specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against NONO. NONO protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against NONO in HepG2 whole cell lysate using NONO specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against NONO. NONO protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHLV17-61

Status: Released

Lab: Graveley Lab

K562 NONO_K562_sc.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using NONO specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-NONO antibody (lanes under 'anti-NONO'). Asterisk indicates heavy and light chains of antibody.

Caption: IP-Western Blot analysis of K562 whole cell lysate using NONO specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-NONO antibody (lanes under 'anti-NONO'). Asterisk indicates heavy and light chains of antibody.

Method: immunoprecipitation

Releated Sample: eCLIP:405, eCLIP:4001

Status: Released

Lab: Fu Lab