Antibodies of NONO


4 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
NONOProduct_ID: A300-587A
Lot_ID: 1
Source: Bethyl Labs
Target Name: NONO-human		 HepG2_Bethyl_A300-587A_1_NONO.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using NONO specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-NONO antibody (lanes under 'NONO').
 NONO_Secondary_Western.png<br>Caption: Western blot following shRNA against NONO in K562 and HepG2 whole cell lysate using NONO specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against NONO. Lanes 5-7 follow the same pattern, but in HepG2. NONO protein appears as the green band, GAPDH serves as a control and appears in red.
 K562_Bethyl_A300-587A_1_NONO.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using NONO specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-NONO antibody (lanes under 'NONO').
 NONO_Secondary_Western.png<br>Caption: Western blot following shRNA against NONO in K562 and HepG2 whole cell lysate using NONO specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against NONO. Lanes 5-7 follow the same pattern, but in HepG2. NONO protein appears as the green band, GAPDH serves as a control and appears in red.
NONOProduct_ID: MA3-2024
Lot_ID: TA26182
Source: Thermo Fisher
Target Name: NONO-human		 ThermoFisher_MA3-2024_TA26182_NONO.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the NONO specific antibody, MA3-2024. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the NONO-specific antibody, MA3-2024. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
NONOProduct_ID: RN092PW
Lot_ID: 1
Source: MBLI
Target Name: NONO-human		 NONO_HepG2_MBL.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using NONO specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit polyclonal anti-NONO antibody (lanes under 'anti-NONO'). Asterisk indicates heavy chain of antibody.
NONOProduct_ID: sc-166702
Lot_ID: C1810
Source: Santa Cruz Biotech
Target Name: NONO-human		 NONO_HepG2_sc.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using NONO specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-NONO antibody (lanes under 'anti-NONO'). Asterisk indicates heavy and light chains of antibody.
 NONO-HEPG2-FU%27S.png<br>Caption: Western blot following shRNA against NONO in HepG2 whole cell lysate using NONO specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against NONO. NONO protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 NONO_K562_sc.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using NONO specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-NONO antibody (lanes under 'anti-NONO'). Asterisk indicates heavy and light chains of antibody.