HNRNPL antibody: ab6106


Antibody Info
Product_IDab6106
Lot_IDGR30383-24
Host_organismmouse
SourceAbcam
Antibody_DCC_IDENCAB734ADP
Target Info
GeneNameHNRNPL
Phosphate
Target_nameHNRNPL-human
Antibody Info
Product_IDab6106
Lot_IDGR30383-23
Host_organismmouse
SourceAbcam
Antibody_DCC_IDENCAB678XLS
Target Info
GeneNameHNRNPL
Phosphate
Target_nameHNRNPL-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Abcam_ab6106_GR30383-24_HNRNPL.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using HNRNPL specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-HNRNPL antibody (lanes under 'HNRNPL').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using HNRNPL specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-HNRNPL antibody (lanes under 'HNRNPL').

Method: immunoprecipitation

Releated Sample: eCLIP:678

Status: Released

Lab: Yeo Lab

K562 K562_Abcam_ab6106_GR30383-24_HNRNPL.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using HNRNPL specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using mouse monoclonal anti-HNRNPL antibody (lanes under 'HNRNPL').

Caption: IP-Western Blot analysis of K562 whole cell lysate using HNRNPL specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using mouse monoclonal anti-HNRNPL antibody (lanes under 'HNRNPL').

Method: immunoprecipitation

Releated Sample: eCLIP:560

Status: Released

Lab: Yeo Lab

Cell linePrimary validationSecondary validation
HepG2 HNRNPL-HEPG2.png<br>Caption: Western blot following shRNA against HNRNPL in HepG2 whole cell lysate using HNRNPL specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against HNRNPL. HNRNPL protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.

Caption: Western blot following shRNA against HNRNPL in HepG2 whole cell lysate using HNRNPL specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against HNRNPL. HNRNPL protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.

Releated Sample: BGHLV23-21

Status: Released

Lab: Graveley Lab

K562 Abcam_ab6106_GR30383-23_HNRNPL.png<br>Caption: IP-WB analysis of K562 whole cell lysate using HNRNPL specific antibody. Lane 1 is 2.5% of five million whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using mouse monoclonal Anti-hnRNP L antibody [4D11]. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.

Caption: IP-WB analysis of K562 whole cell lysate using HNRNPL specific antibody. Lane 1 is 2.5% of five million whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using mouse monoclonal Anti-hnRNP L antibody [4D11]. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.

Method: immunoprecipitation

Releated Sample: eCLIP:560

Status: Released

Lab: Yeo Lab

 HNRNPL-K562.png<br>Caption: Western blot following shRNA against HNRNPL in K562 whole cell lysate using HNRNPL specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against HNRNPL. HNRNPL protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.

Caption: Western blot following shRNA against HNRNPL in K562 whole cell lysate using HNRNPL specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against HNRNPL. HNRNPL protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.

Releated Sample: BGKLV25-25

Status: Released

Lab: Graveley Lab