UPF1 antibody: RN108PW


Antibody Info
Product_IDRN108PW
Lot_ID1
Host_organismrabbit
SourceMBLI
Antibody_DCC_IDENCAB730BOO
Target Info
GeneNameUPF1
Phosphate
Target_nameUPF1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_MBLI_RN108PW_001_UPF1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using UPF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-UPF1 antibody (lanes under 'UPF1').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using UPF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-UPF1 antibody (lanes under 'UPF1').

Method: immunoprecipitation

Releated Sample: eCLIP:726

Status: Released

Lab: Yeo Lab

UPF1-HEPG2.png<br>Caption: Western blot following shRNA against UPF1 in HepG2 whole cell lysate using UPF1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against UPF1. UPF1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against UPF1 in HepG2 whole cell lysate using UPF1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against UPF1. UPF1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHLV33-33

Status: Released

Lab: Graveley Lab

K562 K562_MBLI_RN108PW_001_UPF1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using UPF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-UPF1 antibody (lanes under 'UPF1').

Caption: IP-Western Blot analysis of K562 whole cell lysate using UPF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-UPF1 antibody (lanes under 'UPF1').

Method: immunoprecipitation

Releated Sample: eCLIP:465

Status: Released

Lab: Yeo Lab

UPF1-K562.png<br>Caption: Western blot following shRNA against UPF1 in K562 whole cell lysate using UPF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against UPF1. UPF1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against UPF1 in K562 whole cell lysate using UPF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against UPF1. UPF1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGKLV34-43

Status: Released

Lab: Graveley Lab