Antibodies of UPF1


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
UPF1Product_ID: RN108PW
Lot_ID: 1
Source: MBLI
Target Name: UPF1-human		 HepG2_MBLI_RN108PW_001_UPF1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using UPF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-UPF1 antibody (lanes under 'UPF1').
 UPF1-HEPG2.png<br>Caption: Western blot following shRNA against UPF1 in HepG2 whole cell lysate using UPF1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against UPF1. UPF1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 K562_MBLI_RN108PW_001_UPF1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using UPF1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-UPF1 antibody (lanes under 'UPF1').
 UPF1-K562.png<br>Caption: Western blot following shRNA against UPF1 in K562 whole cell lysate using UPF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against UPF1. UPF1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
UPF1Product_ID: 12040S
Lot_ID: 3
Source: Cell Signaling Technology
Target Name: UPF1-human		 CST_12040S_3_UPF1.png<br>Caption: IP-WB analysis of 12040S whole cell lysate using the UPF1 specific antibody, 12040S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the UPF1-specific antibody, 12040S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 UPF1-K562-12040S.png<br>Caption: Western blot following shRNA against UPF1 in K562 whole cell lysate using UPF1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against UPF1. UPF1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.