HepG2 | ![HepG2_Bethyl_A303-950A_1_SF3B4.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using SF3B4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SF3B4 antibody (lanes under 'SF3B4').](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot_primary/HepG2_Bethyl_A303-950A_1_SF3B4.png)
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using SF3B4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SF3B4 antibody (lanes under 'SF3B4'). Method: immunoprecipitation Releated Sample: eCLIP:228 Status: Released Lab: Yeo Lab | ![SF3B4-HEPG2_Secondary_Western.png<br>Caption: Western blot following shRNA against SF3B4 in HepG2 whole cell lysate using SF3B4 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SF3B4. SF3B4 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot/SF3B4-HEPG2_Secondary_Western.png)
Caption: Western blot following shRNA against SF3B4 in HepG2 whole cell lysate using SF3B4 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SF3B4. SF3B4 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Releated Sample: BGHLV12-73 Status: Released Lab: Graveley Lab |
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K562 | ![k562_bethyl_A303-950A_1_SF3B4.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using SF3B4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SF3B4 antibody (lanes under 'SF3B4').](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot_primary/k562_bethyl_A303-950A_1_SF3B4.png)
Caption: IP-Western Blot analysis of K562 whole cell lysate using SF3B4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SF3B4 antibody (lanes under 'SF3B4'). Method: immunoprecipitation Releated Sample: eCLIP:507 Status: Released Lab: Yeo Lab | ![SF3B4-K562_Secondary_Western.png<br>Caption: Western blot following shRNA against SF3B4 in K562 whole cell lysate using SF3B4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SF3B4. SF3B4 protein appears as the green band, GAPDH serves as a control and appears in red.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot/SF3B4-K562_Secondary_Western.png)
Caption: Western blot following shRNA against SF3B4 in K562 whole cell lysate using SF3B4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SF3B4. SF3B4 protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV08-51 Status: Released Lab: Graveley Lab |
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