SF3B4 antibody: A303-950A


Antibody Info
Product_IDA303-950A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB647CFU
Target Info
GeneNameSF3B4
Phosphate
Target_nameSF3B4-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A303-950A_1_SF3B4.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using SF3B4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SF3B4 antibody (lanes under 'SF3B4').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using SF3B4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SF3B4 antibody (lanes under 'SF3B4').

Method: immunoprecipitation

Releated Sample: eCLIP:228

Status: Released

Lab: Yeo Lab

SF3B4-HEPG2_Secondary_Western.png<br>Caption: Western blot following shRNA against SF3B4 in HepG2 whole cell lysate using SF3B4 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SF3B4. SF3B4 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against SF3B4 in HepG2 whole cell lysate using SF3B4 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SF3B4. SF3B4 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHLV12-73

Status: Released

Lab: Graveley Lab

K562 k562_bethyl_A303-950A_1_SF3B4.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using SF3B4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SF3B4 antibody (lanes under 'SF3B4').

Caption: IP-Western Blot analysis of K562 whole cell lysate using SF3B4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SF3B4 antibody (lanes under 'SF3B4').

Method: immunoprecipitation

Releated Sample: eCLIP:507

Status: Released

Lab: Yeo Lab

SF3B4-K562_Secondary_Western.png<br>Caption: Western blot following shRNA against SF3B4 in K562 whole cell lysate using SF3B4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SF3B4. SF3B4 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against SF3B4 in K562 whole cell lysate using SF3B4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SF3B4. SF3B4 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV08-51

Status: Released

Lab: Graveley Lab