FASTKD2 antibody: A303-788A


Antibody Info
Product_IDA303-788A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB541LFT
Target Info
GeneNameFASTKD2
Phosphate
Target_nameFASTKD2-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A303-788A_1_FASTKD2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using FASTKD2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FASTKD2 antibody (lanes under 'FASTKD2').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using FASTKD2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FASTKD2 antibody (lanes under 'FASTKD2').

Method: immunoprecipitation

Releated Sample: eCLIP:649

Status: Released

Lab: Yeo Lab

FASTKD2_Secondary_Western.png<br>Caption: Western blot following shRNA against FASTKD2 in K562 and HepG2 whole cell lysate using FASTKD2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FASTKD2. Lanes 5-8 follow the same pattern, but in HepG2. FASTKD2 protein appears as the green band, Tubulin or GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against FASTKD2 in K562 and HepG2 whole cell lysate using FASTKD2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FASTKD2. Lanes 5-8 follow the same pattern, but in HepG2. FASTKD2 protein appears as the green band, Tubulin or GAPDH serves as a control and appears in red.

Releated Sample: BGKLV13-53

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A303-788A_1_FASTKD2.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using FASTKD2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FASTKD2 antibody (lanes under 'FASTKD2').

Caption: IP-Western Blot analysis of K562 whole cell lysate using FASTKD2 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FASTKD2 antibody (lanes under 'FASTKD2').

Method: immunoprecipitation

Releated Sample: eCLIP:460

Status: Released

Lab: Yeo Lab

FASTKD2_Secondary_Western.png<br>Caption: Western blot following shRNA against FASTKD2 in K562 and HepG2 whole cell lysate using FASTKD2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FASTKD2. Lanes 5-8 follow the same pattern, but in HepG2. FASTKD2 protein appears as the green band, Tubulin or GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against FASTKD2 in K562 and HepG2 whole cell lysate using FASTKD2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against FASTKD2. Lanes 5-8 follow the same pattern, but in HepG2. FASTKD2 protein appears as the green band, Tubulin or GAPDH serves as a control and appears in red.

Releated Sample: BGKLV13-53

Status: Released

Lab: Graveley Lab