| HepG2 | 
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using TDP43 (TARDBP) specific antibody. Lane 1 is 1% of twenty million whole cell lysate input; lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'lgG'); Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-TDP43 antibody (lanes under 'TDP43'). The expected molecular weight of TDP43 is 43 kDa. Method: immunoprecipitation Releated Sample: eCLIP:4110 Status: Released Lab: Yeo Lab | 
Caption: Western blot following shRNA against TARDBP in HepG2 whole cell lysate using TARDBP specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against TARDBP.TARDBP protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGHLV35-53 Status: Released Lab: Graveley Lab |
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| K562 | 
Caption: IP-Western Blot analysis of K562 whole cell lysate using TARDBP specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-TDP43 antibody (lanes under 'TARDBP'). Comments: This antibody was given and exemption for K562. The data was clean and the pattern is reliable. Method: immunoprecipitation Releated Sample: eCLIP:340, eCLIP:4014 Status: Released Lab: Yeo Lab | 
Caption: Western blot following shRNA against TARDBP in K562 whole cell lysate using TARDBP specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against TARDBP. TARDBP protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV11-57 Status: Released Lab: Graveley Lab |
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