Antibodies of TARDBP


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
TARDBPProduct_ID: A303-223A
Lot_ID: 1
Source: Bethyl Labs
Target Name: TARDBP-human		 HepG2_Bethyl_A303-223A_1_TDP43.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using TDP43 (TARDBP) specific antibody. Lane 1 is 1% of twenty million whole cell lysate input; lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'lgG'); Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-TDP43 antibody (lanes under 'TDP43'). The expected molecular weight of TDP43 is 43 kDa.
 TARDBP-hepg2.png<br>Caption: Western blot following shRNA against TARDBP in HepG2 whole cell lysate using TARDBP specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against TARDBP.TARDBP protein appears as the green band, GAPDH serves as a control and appears in red.
 K562_Bethyl_A303-223A_1_TARDBP.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using TARDBP specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-TDP43 antibody (lanes under 'TARDBP').
 TARDBP_Secondary_Western.png<br>Caption: Western blot following shRNA against TARDBP in K562 whole cell lysate using TARDBP specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against TARDBP. TARDBP protein appears as the green band, GAPDH serves as a control and appears in red.
TARDBPProduct_ID: 711051
Lot_ID: SI259917
Source: Thermo Fisher
Target Name: TARDBP-human		 ThermoFisher_711051_SI259917_TARDBP_HepG2.png<br>Caption: IP-WB analysis of HepG2 whole cell lysate using the TARDBP specific antibody, 711051. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment using a normal IgG antibody, respectively. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the TARDBP-specific antibody, 711051. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 ThermoFisher_711051_SI259917_TARDBP.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the TARDBP specific antibody, 711051. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the TARDBP-specific antibody, 711051. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 TARDBP-K562.png<br>Caption: Western blot following shRNA against TARDBP in K562 whole cell lysate using TARDBP specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against TARDBP. TARDBP protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.