| HepG2 | 
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using ZONAB specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-ZONAB antibody (lanes under 'ZONAB'). Comments: The expected size is 40kDa. In the submitted blot, the major immunoreactive band appears to be outside of the allowed 20% size deviation. Method: immunoprecipitation Releated Sample: eCLIP:729 Status: Released Lab: Yeo Lab | 
Caption: Western blot following shRNA against YBX3 in K562 and HepG2 whole cell lysate using YBX3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against YBX3. Lanes 5-8 follow the same pattern, but in HepG2. YBX3 protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV24-11 Status: Released Lab: Graveley Lab |
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| K562 | 
Caption: IP-Western Blot analysis of K562 whole cell lysate using PRPF8 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-PRPF8 antibody (lanes under 'PRPF8'). Method: immunoprecipitation Releated Sample: eCLIP:551 Status: Released Lab: Yeo Lab |
Caption: Western blot following shRNA against YBX3 in K562 and HepG2 whole cell lysate using YBX3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against YBX3. Lanes 5-8 follow the same pattern, but in HepG2. YBX3 protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV24-11 Status: Released Lab: Graveley Lab |
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