DDX59 antibody: A303-028A


Antibody Info
Product_IDA303-028A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB112HZS
Target Info
GeneNameDDX59
Phosphate
Target_nameDDX59-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A303-028A_1_DDX59.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DDX59 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX59 antibody (lanes under 'DDX59').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DDX59 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX59 antibody (lanes under 'DDX59').

Method: immunoprecipitation

Releated Sample: eCLIP:603

Status: Released

Lab: Yeo Lab

DDX59-HEPG2.png<br>Caption: Western blot following shRNA against DDX59 in HepG2 whole cell lysate using DDX59 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX59.DDX59 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against DDX59 in HepG2 whole cell lysate using DDX59 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX59.DDX59 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHLV35-15

Status: Released

Lab: Graveley Lab

K562 DDX59-K562-CRISPR.png<br>Caption: Western blot following CRISPR against DDX59 in K562 whole cell lysate using DDX59 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX59.DDX59 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against DDX59 in K562 whole cell lysate using DDX59 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX59.DDX59 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGKcLV08-23

Status: Released

Lab: Graveley Lab