NOLC1 antibody: A302-184A


Antibody Info
Product_IDA302-184A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB287BXF
Target Info
GeneNameNOLC1
Phosphate
Target_nameNOLC1-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A302-184A_1_NOLC1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using NOLC1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-NOLC1 antibody (lanes under 'NOLC1').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using NOLC1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-NOLC1 antibody (lanes under 'NOLC1').

Method: immunoprecipitation

Releated Sample: eCLIP:553

Status: Released

Lab: Yeo Lab

NOLC1-CRISPR-HEPG2.png<br>Caption: Western blot following CRISPR against NOLC1 in HepG2 whole cell lysate using NOLC1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NOLC1. NOLC1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against NOLC1 in HepG2 whole cell lysate using NOLC1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NOLC1. NOLC1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHcLV07-19

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A302-184A_1_NOLC1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using NOLC1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-NOLC1 antibody (lanes under 'NOLC1').

Caption: IP-Western Blot analysis of K562 whole cell lysate using NOLC1 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-NOLC1 antibody (lanes under 'NOLC1').

Method: immunoprecipitation

Releated Sample: eCLIP:516

Status: Released

Lab: Yeo Lab