TBRG4 antibody: A301-392A


Antibody Info
Product_IDA301-392A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB890JXZ
Target Info
GeneNameTBRG4
Phosphate
Target_nameTBRG4-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A301-392A_1_TBRG4.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using TBRG4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-TBRG4 antibody (lanes under 'TBRG4').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using TBRG4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-TBRG4 antibody (lanes under 'TBRG4').

Method: immunoprecipitation

Releated Sample: eCLIP:617

Status: Released

Lab: Yeo Lab

TBRG4-HEPG2_Secondary_Western.png<br>Caption: Western blot following shRNA against TBRG4 in HepG2 whole cell lysate using TBRG4 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against TBRG4.TBRG4 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against TBRG4 in HepG2 whole cell lysate using TBRG4 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against TBRG4.TBRG4 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGHLV27-55

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A301-392A_1_TBRG4.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using TBRG4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-TBRG4 antibody (lanes under 'TBRG4').

Caption: IP-Western Blot analysis of K562 whole cell lysate using TBRG4 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-TBRG4 antibody (lanes under 'TBRG4').

Method: immunoprecipitation

Releated Sample: eCLIP:584

Status: Released

Lab: Yeo Lab

TBRG4_Secondary_Western.png<br>Caption: Western blot following shRNA against TBRG4 in K562 whole cell lysate using TBRG4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against TBRG4. TBRG4 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against TBRG4 in K562 whole cell lysate using TBRG4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against TBRG4. TBRG4 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV08_59

Status: Released

Lab: Graveley Lab