SFPQ antibody: A301-321A


Antibody Info
Product_IDA301-321A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB692IKS
Target Info
GeneNameSFPQ
Phosphate
Target_nameSFPQ-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A301-321A_1_SFPQ.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using SFPQ specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SFPQ antibody (lanes under 'SFPQ').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using SFPQ specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SFPQ antibody (lanes under 'SFPQ').

Method: immunoprecipitation

Releated Sample: eCLIP:437

Status: Released

Lab: Yeo Lab

SFPQ_Secondary_Western.png<br>Caption: Western blot following shRNA against SFPQ in K562 and HepG2 whole cell lysate using SFPQ specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SFPQ. Lanes 5-8 follow the same pattern, but in HepG2. SFPQ protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against SFPQ in K562 and HepG2 whole cell lysate using SFPQ specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SFPQ. Lanes 5-8 follow the same pattern, but in HepG2. SFPQ protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV28-51

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A301-321A_1_SFPQ.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using SFPQ specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SFPQ antibody (lanes under 'SFPQ').

Caption: IP-Western Blot analysis of K562 whole cell lysate using SFPQ specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SFPQ antibody (lanes under 'SFPQ').

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab

SFPQ_Secondary_Western.png<br>Caption: Western blot following shRNA against SFPQ in K562 and HepG2 whole cell lysate using SFPQ specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SFPQ. Lanes 5-8 follow the same pattern, but in HepG2. SFPQ protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against SFPQ in K562 and HepG2 whole cell lysate using SFPQ specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SFPQ. Lanes 5-8 follow the same pattern, but in HepG2. SFPQ protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV28-51

Status: Released

Lab: Graveley Lab