HepG2 | ![HepG2_Bethyl_A301-321A_1_SFPQ.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using SFPQ specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SFPQ antibody (lanes under 'SFPQ').](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot_primary/HepG2_Bethyl_A301-321A_1_SFPQ.png)
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using SFPQ specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SFPQ antibody (lanes under 'SFPQ'). Method: immunoprecipitation Releated Sample: eCLIP:437 Status: Released Lab: Yeo Lab | ![SFPQ_Secondary_Western.png<br>Caption: Western blot following shRNA against SFPQ in K562 and HepG2 whole cell lysate using SFPQ specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SFPQ. Lanes 5-8 follow the same pattern, but in HepG2. SFPQ protein appears as the green band, Tubulin serves as a control and appears in red.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot/SFPQ_Secondary_Western.png)
Caption: Western blot following shRNA against SFPQ in K562 and HepG2 whole cell lysate using SFPQ specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SFPQ. Lanes 5-8 follow the same pattern, but in HepG2. SFPQ protein appears as the green band, Tubulin serves as a control and appears in red. Releated Sample: BGKLV28-51 Status: Released Lab: Graveley Lab |
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K562 | ![K562_Bethyl_A301-321A_1_SFPQ.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using SFPQ specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SFPQ antibody (lanes under 'SFPQ').](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot_primary/K562_Bethyl_A301-321A_1_SFPQ.png)
Caption: IP-Western Blot analysis of K562 whole cell lysate using SFPQ specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-SFPQ antibody (lanes under 'SFPQ'). Method: immunoprecipitation Status: Released Lab: Yeo Lab | ![SFPQ_Secondary_Western.png<br>Caption: Western blot following shRNA against SFPQ in K562 and HepG2 whole cell lysate using SFPQ specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SFPQ. Lanes 5-8 follow the same pattern, but in HepG2. SFPQ protein appears as the green band, Tubulin serves as a control and appears in red.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot/SFPQ_Secondary_Western.png)
Caption: Western blot following shRNA against SFPQ in K562 and HepG2 whole cell lysate using SFPQ specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against SFPQ. Lanes 5-8 follow the same pattern, but in HepG2. SFPQ protein appears as the green band, Tubulin serves as a control and appears in red. Releated Sample: BGKLV28-51 Status: Released Lab: Graveley Lab |
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