RBM15 antibody: A300-821A


Antibody Info
Product_IDA300-821A
Lot_ID2
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB626AJI
Target Info
GeneNameRBM15
Phosphate
Target_nameRBM15-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A300-821A_2_RBM15.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using RBM15 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-RBM15 antibody (lanes under 'RBM15').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using RBM15 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-RBM15 antibody (lanes under 'RBM15').

Method: immunoprecipitation

Releated Sample: eCLIP:552

Status: Released

Lab: Yeo Lab

RBM15-hepg2.png<br>Caption: Western blot following shRNA against RBM15 in HepG2 whole cell lysate using RBM15 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RBM15. RBM15 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against RBM15 in HepG2 whole cell lysate using RBM15 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RBM15. RBM15 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHLV31-53

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A300-821A_2_RBM15.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using RBM15 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-RBM15 antibody (lanes under 'RBM15').

Caption: IP-Western Blot analysis of K562 whole cell lysate using RBM15 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-RBM15 antibody (lanes under 'RBM15').

Method: immunoprecipitation

Releated Sample: eCLIP:514

Status: Released

Lab: Yeo Lab

RBM15-K562_Secondary_Western.png<br>Caption: Western blot following shRNA against RBM15 in K562 whole cell lysate using RBM15 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RBM15.RBM15 protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against RBM15 in K562 whole cell lysate using RBM15 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RBM15.RBM15 protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV32-53

Status: Released

Lab: Graveley Lab