Antibodies of RBM15


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RBM15Product_ID: A300-821A
Lot_ID: 2
Source: Bethyl Labs
Target Name: RBM15-human
HepG2_Bethyl_A300-821A_2_RBM15.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using RBM15 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-RBM15 antibody (lanes under 'RBM15').
RBM15-hepg2.png<br>Caption: Western blot following shRNA against RBM15 in HepG2 whole cell lysate using RBM15 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RBM15. RBM15 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
K562_Bethyl_A300-821A_2_RBM15.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using RBM15 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-RBM15 antibody (lanes under 'RBM15').
RBM15-K562_Secondary_Western.png<br>Caption: Western blot following shRNA against RBM15 in K562 whole cell lysate using RBM15 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against RBM15.RBM15 protein appears as the green band, Tubulin serves as a control and appears in red.
RBM15Product_ID: 60386S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: RBM15-human
CST_60386S_1_RBM15.png<br>Caption: IP-WB analysis of 60386S whole cell lysate using the RBM15 specific antibody, 60386S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RBM15-specific antibody, 60386S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.