DDX6 antibody: A300-460A


Antibody Info
Product_IDA300-460A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB348UON
Target Info
GeneNameDDX6
Phosphate
Target_nameDDX6-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A300-406A_1_DDX6.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DDX6 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX6 antibody (lanes under 'DDX6').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DDX6 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX6 antibody (lanes under 'DDX6').

Method: immunoprecipitation

Releated Sample: eCLIP:589

Status: Released

Lab: Yeo Lab

DDX6-HEPG2.png<br>Caption: Western blot following shRNA against DDX6 in HepG2 whole cell lysate using DDX6 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX6. DDX6 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against DDX6 in HepG2 whole cell lysate using DDX6 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX6. DDX6 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHLV12-17

Status: Released

Lab: Graveley Lab

K562 K562_Bethyl_A300-406A_1_DDX6.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using DDX6 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX6 antibody (lanes under 'DDX6').

Caption: IP-Western Blot analysis of K562 whole cell lysate using DDX6 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX6 antibody (lanes under 'DDX6').

Method: immunoprecipitation

Releated Sample: eCLIP:577

Status: Released

Lab: Yeo Lab

DDX6-K562-CRISPR.png<br>Caption: Western blot following CRISPR against DDX6 in K562 whole cell lysate using DDX6 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX6.DDX6 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against DDX6 in K562 whole cell lysate using DDX6 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX6.DDX6 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGKcLV08-29

Status: Released

Lab: Graveley Lab