| HepG2 | 
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using FUS specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FUS antibody (lanes under 'FUS'). Method: immunoprecipitation Releated Sample: eCLIP:694 Status: Released Lab: Yeo Lab | 
Caption: Western blot following shRNA against FUS in K562 and HepG2 whole cell lysate using FUS specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against FUS. Lanes 5-7 follow the same pattern, but in HepG2. FUS protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV13-51 Status: Released Lab: Graveley Lab |
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| K562 | 
Caption: IP-Western blot analysis of K562 whole cell lysate using FUS specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal FUS antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation. Method: immunoprecipitation Releated Sample: eCLIP:689 Status: Released Lab: Yeo Lab |
Caption: Western blot following shRNA against FUS in K562 and HepG2 whole cell lysate using FUS specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against FUS. Lanes 5-7 follow the same pattern, but in HepG2. FUS protein appears as the green band, GAPDH serves as a control and appears in red. Releated Sample: BGKLV13-51 Status: Released Lab: Graveley Lab |
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