Antibodies of FUS


3 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
FUSProduct_ID: A300-294A
Lot_ID: 2
Source: Bethyl Labs
Target Name: FUS-human		 HepG2_Bethyl_A300-294A_2_FUS.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using FUS specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-FUS antibody (lanes under 'FUS').
 FUS_Secondary_Western.png<br>Caption: Western blot following shRNA against FUS in K562 and HepG2 whole cell lysate using FUS specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against FUS. Lanes 5-7 follow the same pattern, but in HepG2. FUS protein appears as the green band, GAPDH serves as a control and appears in red.
 Bethyl_A300-294A_2_FUS.png<br>Caption: IP-Western blot analysis of K562 whole cell lysate using FUS specific antibody. Lane 1 is 2.5% of 0.5mg input lysate, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal FUS antibody. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
 FUS_Secondary_Western.png<br>Caption: Western blot following shRNA against FUS in K562 and HepG2 whole cell lysate using FUS specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 2 and 3 are two different shRNAs against FUS. Lanes 5-7 follow the same pattern, but in HepG2. FUS protein appears as the green band, GAPDH serves as a control and appears in red.
FUSProduct_ID: PA5-52610
Lot_ID: UA2701853A
Source: Thermo Fisher
Target Name: FUS-human		 ThermoFisher_PA5-52610_UA2701853A_FUS.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the FUS specific antibody, PA5-52610. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the FUS-specific antibody, PA5-52610. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
FUSProduct_ID: 67840S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: FUS-human		 CST_67840S_1_FUS.png<br>Caption: IP-WB analysis of 67840S whole cell lysate using the FUS specific antibody, 67840S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the FUS-specific antibody, 67840S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.