QKI antibody: A300-183A


Antibody Info
Product_IDA300-183A
Lot_ID2
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB494QSS
Target Info
GeneNameQKI
Phosphate
Target_nameQKI-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 HepG2_Bethyl_A300-183A_2_QK1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using QKI specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-QKI antibody (lanes under 'QKI').

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using QKI specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-QKI antibody (lanes under 'QKI').

Method: immunoprecipitation

Releated Sample: eCLIP:478

Status: Released

Lab: Yeo Lab

QKI_Secondary_Western.png<br>Caption: Western blot following shRNA against QKI in K562 and HepG2 whole cell lysate using QKI specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against QKI. Lanes 5-8 follow the same pattern, but in HepG2. QKI protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against QKI in K562 and HepG2 whole cell lysate using QKI specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against QKI. Lanes 5-8 follow the same pattern, but in HepG2. QKI protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV08-39

Status: Released

Lab: Graveley Lab

K562 k562_bethyl_A300-183A_2_QK1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using QKI specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-QKI antibody (lanes under 'QKI').

Caption: IP-Western Blot analysis of K562 whole cell lysate using QKI specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-QKI antibody (lanes under 'QKI').

Method: immunoprecipitation

Releated Sample: eCLIP:466

Status: Released

Lab: Yeo Lab

QKI_Secondary_Western.png<br>Caption: Western blot following shRNA against QKI in K562 and HepG2 whole cell lysate using QKI specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against QKI. Lanes 5-8 follow the same pattern, but in HepG2. QKI protein appears as the green band, Tubulin serves as a control and appears in red.

Caption: Western blot following shRNA against QKI in K562 and HepG2 whole cell lysate using QKI specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against QKI. Lanes 5-8 follow the same pattern, but in HepG2. QKI protein appears as the green band, Tubulin serves as a control and appears in red.

Releated Sample: BGKLV08-39

Status: Released

Lab: Graveley Lab