Antibodies of QKI


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
QKIProduct_ID: A300-183A
Lot_ID: 2
Source: Bethyl Labs
Target Name: QKI-human		 HepG2_Bethyl_A300-183A_2_QK1.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using QKI specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-QKI antibody (lanes under 'QKI').
 QKI_Secondary_Western.png<br>Caption: Western blot following shRNA against QKI in K562 and HepG2 whole cell lysate using QKI specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against QKI. Lanes 5-8 follow the same pattern, but in HepG2. QKI protein appears as the green band, Tubulin serves as a control and appears in red.
 k562_bethyl_A300-183A_2_QK1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using QKI specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-QKI antibody (lanes under 'QKI').
 QKI_Secondary_Western.png<br>Caption: Western blot following shRNA against QKI in K562 and HepG2 whole cell lysate using QKI specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against QKI. Lanes 5-8 follow the same pattern, but in HepG2. QKI protein appears as the green band, Tubulin serves as a control and appears in red.
QKIProduct_ID: 23065S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: QKI-human		 CST_23065S_1_QKI.png<br>Caption: IP-WB analysis of 23065S whole cell lysate using the QKI specific antibody, 23065S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the QKI-specific antibody, 23065S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 QKI-K562-23065S.png<br>Caption: Western blot following shRNA against QKI in K562 whole cell lysate using QKI specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against QKI. QKI protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.