DROSHA antibody: 3364


Antibody Info
Product_ID3364
Lot_ID3
Host_organismrabbit
SourceCell Signaling Technology
Antibody_DCC_IDENCAB953TNR
Target Info
GeneNameDROSHA
Phosphate
Target_nameDROSHA-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 DROSHA_HepG2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DROSHA specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '30% input') , lane 2 is 10% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 10% IP enrichment using rabbit monoclonal anti-DROSHA antibody (lanes under 'anti-DROSHA'). Asterisk indicates heavy chain of antibody.

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DROSHA specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '30% input') , lane 2 is 10% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 10% IP enrichment using rabbit monoclonal anti-DROSHA antibody (lanes under 'anti-DROSHA'). Asterisk indicates heavy chain of antibody.

Method: immunoprecipitation

Releated Sample: eCLIP:575

Status: Released

Lab: Fu Lab

 DROSHA-HEPG2-CRISPR.png<br>Caption: Western blot following CRISPR against DROSHA in HepG2 whole cell lysate using DROSHA specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DROSHA. DROSHA protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against DROSHA in HepG2 whole cell lysate using DROSHA specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DROSHA. DROSHA protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHcLV05-13

Status: Released

Lab: Graveley Lab

K562 DROSHA_K562.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using DROSHA specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '30% input') , lane 2 is 10% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 10% IP enrichment using rabbit monoclonal anti-DROSHA antibody (lanes under 'anti-DROSHA'). Asterisk indicates heavy chain of antibody.

Caption: IP-Western Blot analysis of K562 whole cell lysate using DROSHA specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '30% input') , lane 2 is 10% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 10% IP enrichment using rabbit monoclonal anti-DROSHA antibody (lanes under 'anti-DROSHA'). Asterisk indicates heavy chain of antibody.

Method: immunoprecipitation

Releated Sample: eCLIP:481

Status: Released

Lab: Fu Lab