Antibodies of DROSHA


3 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
DROSHAProduct_ID: A301-886A
Lot_ID: 1
Source: Bethyl Labs
Target Name: DROSHA-human		 HepG2_A301-886A_1_DROSHA_MassSpec.png<br>Caption: Representative image of immunoprecipitation performed on whole cell extracts from the HepG2 cell line using the DROSHA-specific antibody A301-886A. Lane 1: Input from IP using control IgG. Lane 2: Immunoprecipitated material using control IgG. Lane 3: Input from IP using DROSHA antibody. Lane 4: Immunoprecipitated material using DROSHA antibody. Outlined regions were excised from gel and subjected to analysis by mass spectrometry. Target molecular weight: 159.32 kDa.
 DROSHA%20HepG2.pdf
 K562_Bethyl_A301-886A_1_DROSHA.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using DROSHA specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DROSHA antibody (lanes under 'DROSHA').
 DROSHA-K562_Secondary_Western.png<br>Caption: Western blot following shRNA against DROSHA in K562 whole cell lysate using DROSHA specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DROSHA.DROSHA protein appears as the green band, Tubulin serves as a control and appears in red.
DROSHAProduct_ID: 3364
Lot_ID: 3
Source: Cell Signaling Technology
Target Name: DROSHA-human		 DROSHA_HepG2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DROSHA specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '30% input') , lane 2 is 10% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 10% IP enrichment using rabbit monoclonal anti-DROSHA antibody (lanes under 'anti-DROSHA'). Asterisk indicates heavy chain of antibody.
 DROSHA-HEPG2-CRISPR.png<br>Caption: Western blot following CRISPR against DROSHA in HepG2 whole cell lysate using DROSHA specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DROSHA. DROSHA protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 DROSHA_K562.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using DROSHA specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '30% input') , lane 2 is 10% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 10% IP enrichment using rabbit monoclonal anti-DROSHA antibody (lanes under 'anti-DROSHA'). Asterisk indicates heavy chain of antibody.
DROSHAProduct_ID: 3364s
Lot_ID: 5
Source: Cell Signaling Technology
Target Name: DROSHA-human		 CST_3364s_5_DROSHA.png<br>Caption: IP-WB analysis of 3364s whole cell lysate using the DROSHA specific antibody, 3364s. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DROSHA-specific antibody, 3364S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 DROSHA-K562-CRISPR-3364S.png<br>Caption: Western blot following CRISPR against DROSHA in K562 whole cell lysate using DROSHA specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DROSHA. DROSHA protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.