ENCORE Antibody

Product_ID	13113S
Lot_ID	4
Host_organism	Rabbit
Source	Cell Signaling Technology
Antibody_DCC_ID

GeneName	TFRC
Phosphate
Target_name	TFRC-human

Validation Images

Cell line	Primary validation	Secondary validation
HepG2		Caption: Western blot following CRISPR against TFRC in HepG2 whole cell lysate using TFRC specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against TFRC. TFRC protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow. Releated Sample: BGHcLV45-77 Status: Experimented Lab: Graveley Lab
K562	Caption: IP-WB analysis of 13113S whole cell lysate using the TFRC specific antibody, 13113S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the TFRC-specific antibody, 13113S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. NOTE Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used. Method: immunoprecipitation Status: Submitted Lab: Yeo Lab

Cell line

Primary validation

Secondary validation

HepG2

Caption: Western blot following CRISPR against TFRC in HepG2 whole cell lysate using TFRC specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against TFRC. TFRC protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Releated Sample: BGHcLV45-77

Status: Experimented

Lab: Graveley Lab

K562

CST_13113S_4_TFRC.png<br>Caption: IP-WB analysis of 13113S whole cell lysate using the TFRC specific antibody, 13113S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the TFRC-specific antibody, 13113S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Caption: IP-WB analysis of 13113S whole cell lysate using the TFRC specific antibody, 13113S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the TFRC-specific antibody, 13113S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Method: immunoprecipitation

Status: Submitted

Lab: Yeo Lab

TFRC antibody: 13113S