TFRC      CRISPR in HepG2      Batch: BGHcLV45

Experiment Information (Status: Sequencing)
BGHcLV45-77BGHcLV45-77BGHcLV45-78BGHcLV45-78
idx0000
TRCN#_or_BGC#BGC#0001197BGC#0001197BGC#0001197BGC#0001197
shRNA_or_gRNA_sequenceGGCAATCCTGATGACCGAGAGGCAATCCTGATGACCGAGAGGCAATCCTGATGACCGAGAGGCAATCCTGATGACCGAGA
PAMTGGTGGTGGTGG
NameTFRC_86TFRC_86TFRC_86TFRC_86
Sample_IDBGHcLV45-77BGHcLV45-77BGHcLV45-78BGHcLV45-78
transduction_Date10/1/2410/1/2410/1/2410/1/24
daysD6D6D6D6
RBP_nameTFRCTFRCTFRCTFRC
qPCR_result95.597.0
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc2933293325142514
WB_result90.1no clear band on the position85.8
Ave_WB
WB_DONE_date10/25/2411/7/24
MW90kd90kd
IP
antibody_Cat#13113S13208Slot # 4lot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM0000
Rep2_TPM0000
ActionReadyReady
Library_start_date
repeat_library
Note
ID7719772177207722




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
TFRCProduct_ID: 13113S
Lot_ID: 4
Source: Cell Signaling Technology
Target Name: TFRC-human		 TFRC-HEPG2-CRISPR-13113S.png<br>Caption: Western blot following CRISPR against TFRC in HepG2 whole cell lysate using TFRC specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against TFRC. TFRC protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_13113S_4_TFRC.png<br>Caption: IP-WB analysis of 13113S whole cell lysate using the TFRC specific antibody, 13113S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the TFRC-specific antibody, 13113S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.




Experiment Status
BGHcLV45-77BGHcLV45-77BGHcLV45-78BGHcLV45-78
Sample_IDBGHcLV45-77BGHcLV45-77BGHcLV45-78BGHcLV45-78
Sample Name
Sample NameTFRC-BGHcLV45-77TFRC-BGHcLV45-78
RBPTFRCTFRCTFRCTFRC
Cell_LineHepG2HepG2HepG2HepG2
Exp UID
StatusSequencingNotSatisfiedSequencingNotSatisfied
Status_date2024-12-122024-11-252024-12-122024-11-25
ProjectENCORE2ENCORE2ENCORE2ENCORE2
Note
ID13722137701372313771




Library-Prep Information
BGHcLV45-77BGHcLV45-78
Sample #3738
Sample NameTFRC-BGHcLV45-77TFRC-BGHcLV45-78
Sample_Name_Alias
Index Well PositionE11F11
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2024-12-022024-12-02
Lib_IDLib-241202Lib-241202
Tecan_Location
Tecan
Tecan_date
Size_bp290287
Peak_Molarity77.3079.20
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-12-052024-12-05
Sample_IDBGHcLV45-77BGHcLV45-78
RBPTFRCTFRC
Batch_IDBGHcLV45BGHcLV45
WB_result90.10085.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID46464647




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database