Antibodies of DDX47


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
DDX47Product_ID: A302-976A
Lot_ID: 1
Source: Bethyl Labs
Target Name: DDX47-human		 DDX47-HEPG2-A302-976A.png<br>Caption: Western blot following shRNA against DDX47 in HepG2 whole cell lysate using DDX47 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against DDX47. DDX47 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A302-976A_1_DDX47.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the DDX47 specific antibody, A302-976A. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the DDX47-specific antibody, A302-976A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
DDX47Product_ID: GTX120497
Lot_ID: 40387
Source: GeneTex
Target Name: DDX47-human		 DDX47_Secondary_Western.png<br>Caption: Western blot following shRNA against DDX47 in K562 and HepG2 whole cell lysate using DDX47 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX47. Lanes 5-8 follow the same pattern, but in HepG2. DDX47 protein appears as the green band, GAPDH serves as a control and appears in red.
 K562_genetex_GTX120497_40387_DDX47.jpg<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using DDX47 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal Anti-DDX47 antibody (lanes under 'DDX47').
 DDX47_Secondary_Western.png<br>Caption: Western blot following shRNA against DDX47 in K562 and HepG2 whole cell lysate using DDX47 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX47. Lanes 5-8 follow the same pattern, but in HepG2. DDX47 protein appears as the green band, GAPDH serves as a control and appears in red.