DDX47 antibody: GTX120497


Antibody Info
Product_IDGTX120497
Lot_ID40387
Host_organismrabbit
SourceGeneTex
Antibody_DCC_IDENCAB996IKB
Target Info
GeneNameDDX47
Phosphate
Target_nameDDX47-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 DDX47_Secondary_Western.png<br>Caption: Western blot following shRNA against DDX47 in K562 and HepG2 whole cell lysate using DDX47 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX47. Lanes 5-8 follow the same pattern, but in HepG2. DDX47 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against DDX47 in K562 and HepG2 whole cell lysate using DDX47 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX47. Lanes 5-8 follow the same pattern, but in HepG2. DDX47 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV25-13

Status: Released

Lab: Graveley Lab

K562 K562_genetex_GTX120497_40387_DDX47.jpg<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using DDX47 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal Anti-DDX47 antibody (lanes under 'DDX47').

Caption: IP-Western Blot analysis of K562 whole cell lysate using DDX47 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 10% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal Anti-DDX47 antibody (lanes under 'DDX47').

Method: immunoprecipitation

Releated Sample: eCLIP:4050

Status: Released

Lab: Yeo Lab

DDX47_Secondary_Western.png<br>Caption: Western blot following shRNA against DDX47 in K562 and HepG2 whole cell lysate using DDX47 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX47. Lanes 5-8 follow the same pattern, but in HepG2. DDX47 protein appears as the green band, GAPDH serves as a control and appears in red.

Caption: Western blot following shRNA against DDX47 in K562 and HepG2 whole cell lysate using DDX47 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX47. Lanes 5-8 follow the same pattern, but in HepG2. DDX47 protein appears as the green band, GAPDH serves as a control and appears in red.

Releated Sample: BGKLV25-13

Status: Released

Lab: Graveley Lab