DDX47 antibody: A302-976A


Antibody Info
Product_IDA302-976A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB088DCV
Target Info
GeneNameDDX47
Phosphate
Target_nameDDX47-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 DDX47-HEPG2-A302-976A.png<br>Caption: Western blot following shRNA against DDX47 in HepG2 whole cell lysate using DDX47 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against DDX47. DDX47 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Caption: Western blot following shRNA against DDX47 in HepG2 whole cell lysate using DDX47 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against DDX47. DDX47 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Releated Sample: BGHLV22-9

Status: Released

Lab: Graveley Lab

K562 Bethyl_A302-976A_1_DDX47.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the DDX47 specific antibody, A302-976A. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the DDX47-specific antibody, A302-976A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Caption: IP-WB analysis of K562 whole cell lysate using the DDX47 specific antibody, A302-976A. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the DDX47-specific antibody, A302-976A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Method: immunoprecipitation

Releated Sample: eCLIP:4050

Status: Released

Lab: Yeo Lab