Antibodies of CCAR2


5 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CCAR2Product_ID: A300-433A
Lot_ID: 2
Source: Bethyl Labs
Target Name: CCAR2-human		 Bethyl_A300-433A_2_CCAR2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CCAR2 specific antibody, A300-433A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CCAR2-specific antibody, A300-433A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CCAR2-K562-A300-433A.png<br>Caption: Western blot following shRNA against CCAR2 in K562 whole cell lysate using CCAR2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against CCAR2. CCAR2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
CCAR2Product_ID: RN117PW
Lot_ID: 1
Source: MBLI
Target Name: CCAR2-human		 MBL_RN117PW_001_CCAR2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CCAR2 specific antibody, RN117PW. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the CCAR2-specific antibody, RN117PW. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CCAR2-K562-RN117PW.png<br>Caption: Western blot following shRNA against CCAR2 in K562 whole cell lysate using CCAR2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against CCAR2. CCAR2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
CCAR2Product_ID: A300-432A
Lot_ID: 2
Source: Bethyl Labs
Target Name: CCAR2-human		 Bethyl_A300-432A_2_CCAR2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CCAR2 specific antibody, A300-432A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CCAR2-specific antibody, A300-432A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CCAR2-K562-A300-432A.png<br>Caption: Western blot following shRNA against CCAR2 in K562 whole cell lysate using CCAR2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against CCAR2. CCAR2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
CCAR2Product_ID: 200093
Lot_ID: AD0810
Source: Zen Bioscience
Target Name: CCAR2-human		 DBC1_HepG2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DBC1 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-DBC1 antibody (lanes under 'DBC1'). Asterisk indicates heavy and light chains of antibody.
 DBC1-HEPG2_FU's.png<br>Caption: Western blot following shRNA against DBC1 in HepG2 whole cell lysate using DBC1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against DBC1. DBC1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 DBC1_K562.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using DBC1 specific antibody. Lane 1 is 4% of ten million whole cell lysate input (lane under '20% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-DBC1 antibody (lanes under 'anti-DBC1').
CCAR2Product_ID: A303-942A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CCAR2-human		 DBC1_Secondary_Western.png<br>Caption: Western blot following shRNA against DBC1 in K562 and HepG2 whole cell lysate using DBC1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DBC1. Lanes 5-8 follow the same pattern, but in HepG2. DBC1 protein appears as the red band, GAPDH serves as a control and appears in green.
 DBC1_Secondary_Western.png<br>Caption: Western blot following shRNA against DBC1 in K562 and HepG2 whole cell lysate using DBC1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DBC1. Lanes 5-8 follow the same pattern, but in HepG2. DBC1 protein appears as the red band, GAPDH serves as a control and appears in green.