| K562 | 
Caption: IP-WB analysis of K562 whole cell lysate using the CCAR2 specific antibody, RN117PW. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the CCAR2-specific antibody, RN117PW. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used. Comments: Immunoprecipitation lane (3) exhibits a large deviation from the expected protein size, which we believe is due to performing the characterization using the Jess Western Blotting system. This system has a documented tendency for some proteins to run differently than with more traditional methods due to their compositions and interactions with the electrophoretic components. Method: immunoprecipitation Status: NotSatisfied Lab: Yeo Lab | 
Caption: Western blot following shRNA against CCAR2 in K562 whole cell lysate using CCAR2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against CCAR2. CCAR2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Releated Sample: BGKLV25-29 Status: Released Lab: Graveley Lab |
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