HepG2 | ![DBC1_HepG2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DBC1 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-DBC1 antibody (lanes under 'DBC1'). Asterisk indicates heavy and light chains of antibody.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot_primary/DBC1_HepG2.png)
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DBC1 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-DBC1 antibody (lanes under 'DBC1'). Asterisk indicates heavy and light chains of antibody. Method: immunoprecipitation Status: Released Lab: Fu Lab | ![DBC1-HEPG2_FU's.png<br>Caption: Western blot following shRNA against DBC1 in HepG2 whole cell lysate using DBC1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against DBC1. DBC1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot/DBC1-HEPG2_FU's.png)
Caption: Western blot following shRNA against DBC1 in HepG2 whole cell lysate using DBC1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against DBC1. DBC1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Status: Released Lab: Graveley Lab |
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K562 | ![DBC1_K562.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using DBC1 specific antibody. Lane 1 is 4% of ten million whole cell lysate input (lane under '20% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-DBC1 antibody (lanes under 'anti-DBC1').](https://graveleylab.cam.uchc.edu/ENCODE/ENCODE_DATA/WesternBlot_primary/DBC1_K562.png)
Caption: IP-Western Blot analysis of K562 whole cell lysate using DBC1 specific antibody. Lane 1 is 4% of ten million whole cell lysate input (lane under '20% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-DBC1 antibody (lanes under 'anti-DBC1'). Comments: genecards says Mass (Da): 102,902 Method: immunoprecipitation Status: Released Lab: Fu Lab | |
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