CCAR2 antibody: 200093


Antibody Info
Product_ID200093
Lot_IDAD0810
Host_organismmouse
SourceZen Bioscience
Antibody_DCC_IDENCAB005NAZ
Target Info
GeneNameCCAR2
Phosphate
Target_nameCCAR2-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 DBC1_HepG2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DBC1 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-DBC1 antibody (lanes under 'DBC1'). Asterisk indicates heavy and light chains of antibody.

Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DBC1 specific antibody. Lane 1 is 2% of ten million whole cell lysate input (lane under '10% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-DBC1 antibody (lanes under 'DBC1'). Asterisk indicates heavy and light chains of antibody.

Method: immunoprecipitation

Status: Released

Lab: Fu Lab

DBC1-HEPG2_FU's.png<br>Caption: Western blot following shRNA against DBC1 in HepG2 whole cell lysate using DBC1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against DBC1. DBC1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following shRNA against DBC1 in HepG2 whole cell lysate using DBC1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different shRNA against DBC1. DBC1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Status: Released

Lab: Graveley Lab

K562 DBC1_K562.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using DBC1 specific antibody. Lane 1 is 4% of ten million whole cell lysate input (lane under '20% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-DBC1 antibody (lanes under 'anti-DBC1').

Caption: IP-Western Blot analysis of K562 whole cell lysate using DBC1 specific antibody. Lane 1 is 4% of ten million whole cell lysate input (lane under '20% input') , lane 2 is 20% of IP enrichment using mouse normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using mouse monoclonal anti-DBC1 antibody (lanes under 'anti-DBC1').

Comments: genecards says Mass (Da): 102,902

Method: immunoprecipitation

Status: Released

Lab: Fu Lab