non-target      CRISPR in K562      Control: NT-BGKcLV44-1,NT-BGKcLV44-2

General Information

List KD experiments controlled by BGKcLV44-1 and BGKcLV44-2


NT-BGKcLV44-1,NT-BGKcLV44-2:
  • ALYREF-BGKcLV44-K562
  • CPSF1-BGKcLV44-K562
  • DEK-BGKcLV44-K562
  • ESS2-BGKcLV44-K562
  • EIF2S1-BGKcLV44-K562
  • EIF5B-BGKcLV44-K562
  • NFX1-BGKcLV44-K562
  • NSD2-BGKcLV44-K562
  • PABPN1-BGKcLV44-K562
  • PRPF39-BGKcLV44-K562
  • RACK1-BGKcLV44-K562
  • RANGAP1-BGKcLV44-K562
  • RPS15-BGKcLV44-K562
  • SENP3-BGKcLV44-K562
  • SRRT-BGKcLV44-K562
  • SRSF10-BGKcLV44-K562
  • SSR1-BGKcLV44-K562
  • WARS-BGKcLV44-K562
  • YARS-BGKcLV44-K562
  • RBM5-BGKcLV44-K562




    • Experiment Information (Status: Submitting)
    BGKcLV44-1BGKcLV44-2
    idx00
    TRCN#_or_BGC#
    shRNA_or_gRNA_sequence
    PAM
    Namenon-targetnon-target
    Sample_IDBGKcLV44-1BGKcLV44-2
    transduction_Date7/30/247/30/24
    daysD6D6
    RBP_namenon-targetnon-target
    qPCR_result
    Ave_qPCR
    RT-qPCR_primer-F
    RT-qPCR_primer-R
    protein_conc04349
    WB_result
    Ave_WB
    WB_DONE_date
    MW
    IP
    antibody_Cat#
    Antibody DCC ID
    submitted_to_DCC_date
    Rep1_TPM00
    Rep2_TPM00
    ActionReadyReady
    Library_start_date
    repeat_library
    Note
    ID58785879




    Western Blot
    Western Blot info was not avaliable




    Experiment Status
    BGKcLV44-1BGKcLV44-2
    Sample_IDBGKcLV44-1BGKcLV44-2
    Sample Name
    Sample NameNT-BGKcLV44-1NT-BGKcLV44-2
    RBPnon-targetnon-target
    Cell_LineK562K562
    Exp UID
    StatusSubmittingSubmitting
    Status_date2025-02-112025-02-11
    ProjectENCORE2ENCORE2
    Note
    ID1358013581




    Library-Prep Information
    BGKcLV44-1BGKcLV44-2
    Sample #910
    Sample NameNT-BGKcLV44-1NT-BGKcLV44-2
    Sample_Name_Alias
    Index Well PositionA07B07
    Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
    LibPrep_date2024-10-102024-10-10
    Lib_IDLib-241010Lib-241010
    Tecan_Location
    Tecan
    Tecan_date
    Size_bp278288
    Peak_Molarity55.1066.50
    libSampleQC_DNA_WellDNA_Library_set75/set1/A7.pngDNA_Library_set75/set1/B7.png
    RIN10.010.0
    libSample_RNA_WellRNA_Library_set75/set1/A1.pngRNA_Library_set75/set1/B1.png
    SampleQC_methodTapeStation_2022TapeStation_2022
    SampleQC_date2024-10-152024-10-15
    Sample_IDBGKcLV44-1BGKcLV44-2
    RBPNTNT
    Batch_IDBGKcLV44BGKcLV44
    WB_result0.0000.000
    Library DescriptionTruSeq mRNATruSeq mRNA
    Repeat_Library_Suffix
    Lib_StatusSendToSequenceSendToSequence
    ProjectENCORE2ENCORE2
    ID45684569




    Sequencing Information
    BGKcLV44-1BGKcLV44-2
    Sample_IDBGKcLV44-1BGKcLV44-2
    Sample NameNT-BGKcLV44-1NT-BGKcLV44-2
    Pool IDPool-241015Pool-241015
    LocalServer_folderset75set75
    total_reads72,258,33354,452,913
    total_aligned_reads66,616,26651,427,433
    unique_aligned_reads60,985,45347,275,225
    percent_uniqueAligned0.843990.86819
    correlation_replicates0.9889160.988916
    spikein_reads184,530136,080
    percent_spikeins0.002550.00250
    original_ReadLength101101
    QC_StatusSubmittingSubmitting
    ID32793280




    Data Submission Information
    DCC submission information was not avaliable




    File Information
    file_namefile_pathfile_formatoutput_typeassay_typelabSample IDplatformread_lengthrun_typepaired_endassemblygenome_annotationderived_fromcontrolled_byProjectNoteFILE_ID
    NT-BGKcLV44-1_S1_L001_R1_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set75/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3279NovaSeq6000100paired-ended1ENCORE230952
    NT-BGKcLV44-1_S1_L001_R2_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set75/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3279NovaSeq6000100paired-ended2ENCORE230994
    NT-BGKcLV44-1_Aligned.sortedByCoord.out.bamENCODE_DATA/set75/STAR_ENCORE2_trimmed_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab3279GRCh38V40NT-BGKcLV44-1_S1_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV44-1_S1_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE231036
    NT-BGKcLV44-1_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set75/STAR_ENCORE2_trimmed_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3279GRCh38V40NT-BGKcLV44-1_Aligned.sortedByCoord.out.bamENCORE231078
    NT-BGKcLV44-1_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set75/STAR_ENCORE2_trimmed_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3279GRCh38V40NT-BGKcLV44-1_Aligned.sortedByCoord.out.bamENCORE231120
    NT-BGKcLV44-1_Signal.Unique.strand-.bwENCODE_DATA/set75/STAR_ENCORE2_trimmed_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3279GRCh38V40NT-BGKcLV44-1_Aligned.sortedByCoord.out.bamENCORE231162
    NT-BGKcLV44-1_Signal.Unique.strand+.bwENCODE_DATA/set75/STAR_ENCORE2_trimmed_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3279GRCh38V40NT-BGKcLV44-1_Aligned.sortedByCoord.out.bamENCORE231204
    NT-BGKcLV44-1_quant.sfENCODE_DATA/set75/salmon_ENCORE2_file_linkstranscript_quantificationtranscript quantificationsknockdown followed by RNA-seqGraveley Lab3279GRCh38V40NT-BGKcLV44-1_S1_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV44-1_S1_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE231246
    NT-BGKcLV44-2_S2_L001_R1_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set75/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3280NovaSeq6000100paired-ended1ENCORE230953
    NT-BGKcLV44-2_S2_L001_R2_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set75/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3280NovaSeq6000100paired-ended2ENCORE230995
    NT-BGKcLV44-2_Aligned.sortedByCoord.out.bamENCODE_DATA/set75/STAR_ENCORE2_trimmed_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab3280GRCh38V40NT-BGKcLV44-2_S2_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV44-2_S2_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE231037
    NT-BGKcLV44-2_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set75/STAR_ENCORE2_trimmed_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3280GRCh38V40NT-BGKcLV44-2_Aligned.sortedByCoord.out.bamENCORE231079
    NT-BGKcLV44-2_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set75/STAR_ENCORE2_trimmed_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3280GRCh38V40NT-BGKcLV44-2_Aligned.sortedByCoord.out.bamENCORE231121
    NT-BGKcLV44-2_Signal.Unique.strand-.bwENCODE_DATA/set75/STAR_ENCORE2_trimmed_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3280GRCh38V40NT-BGKcLV44-2_Aligned.sortedByCoord.out.bamENCORE231163
    NT-BGKcLV44-2_Signal.Unique.strand+.bwENCODE_DATA/set75/STAR_ENCORE2_trimmed_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3280GRCh38V40NT-BGKcLV44-2_Aligned.sortedByCoord.out.bamENCORE231205
    NT-BGKcLV44-2_quant.sfENCODE_DATA/set75/salmon_ENCORE2_file_linkstranscript_quantificationtranscript quantificationsknockdown followed by RNA-seqGraveley Lab3280GRCh38V40NT-BGKcLV44-2_S2_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV44-2_S2_L001_R2_001.filtered.trimmed.paired.fastq.gzENCORE231247