GTF3C4 RNA-Seq in Ribo-STAMP-293T

Experiment Information (Status: Submitted)

	BGRSDLV02-33	BGRSDLV02-34
idx	0	0
TRCN#_or_BGC#	TRCN0000013400	TRCN0000013400
shRNA_or_gRNA_sequence	CCGGCCATCTCTTCATGCAACACAACTCGAGTTGTGTTGCATGAAGAGATGGTTTTT	CCGGCCATCTCTTCATGCAACACAACTCGAGTTGTGTTGCATGAAGAGATGGTTTTT
PAM
Name	GTF3C4	GTF3C4
Sample_ID	BGRSDLV02-33	BGRSDLV02-34
transduction_Date	9/15/23	9/15/23
days	4 days	4 days
RBP_name	GTF3C4	GTF3C4
qPCR_result	84.2	85.4
Ave_qPCR	84.8
RT-qPCR_primer-F	gctcaacagctgtctcctca
RT-qPCR_primer-R	gcgaatttctccttgcactc
protein_conc	0	0
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	9244	9245

Western Blot Western Blot info was not avaliable

Experiment Status

	BGRSDLV02-33	BGRSDLV02-34
Sample_ID	BGRSDLV02-33	BGRSDLV02-34
Sample Name
Sample Name	GTF3C4-BGRSDLV02-33	GTF3C4-BGRSDLV02-34
RBP	GTF3C4	GTF3C4
Cell_Line	DOX-RS293	DOX-RS293
Exp UID
Status	Submitted	Submitted
Status_date	2024-12-03	2024-12-03
Project	ENCORE2	ENCORE2
Note
ID	12348	12349

Library-Prep
Sequencing

Library-Prep Information

	BGRSDLV02-33	BGRSDLV02-34
Sample #	29	30
Sample Name	GTF3C4-BGRSDLV02-33	GTF3C4-BGRSDLV02-34
Sample_Name_Alias
Index Well Position	E04	F04
Index_table	IDT_UniqueDualIndex_96	IDT_UniqueDualIndex_96
LibPrep_date	2023-09-26	2023-09-26
Lib_ID	Lib-230926	Lib-230926
Tecan_Location
Tecan
Tecan_date
Size_bp	289	291
Peak_Molarity	81.50	92.00
libSampleQC_DNA_Well	DNA_Library_set65/set1/E4.png	DNA_Library_set65/set1/F4.png
RIN	10.0	10.0
libSample_RNA_Well	RNA_Library_set65/set1/E4.png	RNA_Library_set65/set1/F4.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2023-09-27	2023-09-27
Sample_ID	BGRSDLV02-33	BGRSDLV02-34
RBP	GTF3C4	GTF3C4
Batch_ID	BGRSDLV02	BGRSDLV02
WB_result	0.000	0.000
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	3950	3951

Sequencing Information

	BGRSDLV02-33	BGRSDLV02-34
Sample_ID	BGRSDLV02-33	BGRSDLV02-34
Sample Name	GTF3C4-BGRSDLV02-33	GTF3C4-BGRSDLV02-34
Pool ID	Pool-231003	Pool-231003
LocalServer_folder	set65	set65
total_reads	26,779,089	22,018,473
total_aligned_reads	24,484,363	20,248,956
unique_aligned_reads	22,813,164	18,896,156
percent_uniqueAligned	0.85190	0.85820
correlation_replicates	0.995237	0.995237
spikein_reads	2,160	1,586
percent_spikeins	0.00008	0.00007
original_ReadLength	100	100
QC_Status	Submitted	Submitted
ID	2697	2698

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
GTF3C4-BGRSDLV02-33_S29_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set65/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2697	NovaSeq6000	100	paired-ended	1				NT-BGRSDLV02-1_S1_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGRSDLV02-2_S2_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	8766
GTF3C4-BGRSDLV02-33_S29_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set65/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2697	NovaSeq6000	100	paired-ended	2				NT-BGRSDLV02-1_S1_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGRSDLV02-2_S2_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	8842
GTF3C4-BGRSDLV02-33_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set65/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	2697					GRCh38	V40	GTF3C4-BGRSDLV02-33_S29_L001_R1_001.filtered.trimmed.paired.fastq.gz,GTF3C4-BGRSDLV02-33_S29_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	9331
GTF3C4-BGRSDLV02-33_Signal.Unique.strand+.bw	ENCODE_DATA/set65/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2697					GRCh38	V40	GTF3C4-BGRSDLV02-33/Aligned.sortedByCoord.out.bam		ENCORE2	9407
GTF3C4-BGRSDLV02-33_Signal.Unique.strand-.bw	ENCODE_DATA/set65/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2697					GRCh38	V40	GTF3C4-BGRSDLV02-33/Aligned.sortedByCoord.out.bam		ENCORE2	9483
GTF3C4-BGRSDLV02-33_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set65/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2697					GRCh38	V40	GTF3C4-BGRSDLV02-33/Aligned.sortedByCoord.out.bam		ENCORE2	9559
GTF3C4-BGRSDLV02-33_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set65/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2697					GRCh38	V40	GTF3C4-BGRSDLV02-33/Aligned.sortedByCoord.out.bam		ENCORE2	9635
GTF3C4-BGRSDLV02-33_quant.sf	ENCODE_DATA/set65/salmon_ENCORE2	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	2697					GRCh38	V40	GTF3C4-BGRSDLV02-33_S29_L001_R1_001.filtered.trimmed.paired.fastq.gz,GTF3C4-BGRSDLV02-33_S29_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	9711
GTF3C4-BGRSDLV02-34_S30_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set65/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2698	NovaSeq6000	100	paired-ended	1				NT-BGRSDLV02-1_S1_L001_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGRSDLV02-2_S2_L001_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	8767
GTF3C4-BGRSDLV02-34_S30_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set65/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	2698	NovaSeq6000	100	paired-ended	2				NT-BGRSDLV02-1_S1_L001_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGRSDLV02-2_S2_L001_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	8843
GTF3C4-BGRSDLV02-34_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set65/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	2698					GRCh38	V40	GTF3C4-BGRSDLV02-34_S30_L001_R1_001.filtered.trimmed.paired.fastq.gz,GTF3C4-BGRSDLV02-34_S30_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	9332
GTF3C4-BGRSDLV02-34_Signal.Unique.strand+.bw	ENCODE_DATA/set65/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2698					GRCh38	V40	GTF3C4-BGRSDLV02-34/Aligned.sortedByCoord.out.bam		ENCORE2	9408
GTF3C4-BGRSDLV02-34_Signal.Unique.strand-.bw	ENCODE_DATA/set65/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	2698					GRCh38	V40	GTF3C4-BGRSDLV02-34/Aligned.sortedByCoord.out.bam		ENCORE2	9484
GTF3C4-BGRSDLV02-34_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set65/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2698					GRCh38	V40	GTF3C4-BGRSDLV02-34/Aligned.sortedByCoord.out.bam		ENCORE2	9560
GTF3C4-BGRSDLV02-34_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set65/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	2698					GRCh38	V40	GTF3C4-BGRSDLV02-34/Aligned.sortedByCoord.out.bam		ENCORE2	9636
GTF3C4-BGRSDLV02-34_quant.sf	ENCODE_DATA/set65/salmon_ENCORE2	transcript_quantification	transcript quantifications	knockdown followed by RNA-seq	Graveley Lab	2698					GRCh38	V40	GTF3C4-BGRSDLV02-34_S30_L001_R1_001.filtered.trimmed.paired.fastq.gz,GTF3C4-BGRSDLV02-34_S30_L001_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	9712