pum3      CRISPR in K562      Batch: BGKcLV53

Experiment Information (Status: Sequencing)
BGKcLV53-93BGKcLV53-94
idx00
TRCN#_or_BGC#BGC#0001529BGC#0001529
shRNA_or_gRNA_sequenceTGTTCGGGCAAAGCAGATGTTGTTCGGGCAAAGCAGATGT
PAMGGGGGG
Namepum3_76pum3_76
Sample_IDBGKcLV53-93BGKcLV53-94
transduction_Date2/24/262/24/26
daysD6D6
RBP_namepum3pum3
qPCR_result32.827.3
Ave_qPCR30.0
RT-qPCR_primer-Facaggaaagagtacaaagacagc
RT-qPCR_primer-Rccttctttagcaactttccttgt
protein_conc33383203
WB_result74.872.5
Ave_WB
WB_DONE_date3/24/25
MW74kd
IP
antibody_Cat#A303-897Alot #1
Antibody DCC IDENCAB600NAG
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID64556456




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
PUM3Product_ID: A303-897A
Lot_ID: 1
Source: Bethyl Labs
Target Name: PUM3-human		 PUM3-K562-CRISPR-A303-897A.png<br>Caption: Western blot following CRISPR against PUM3 in K562 whole cell lysate using PUM3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PUM3. PUM3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV53-93BGKcLV53-94
Sample_IDBGKcLV53-93BGKcLV53-94
Sample Name
Sample Namepum3-BGKcLV53-93pum3-BGKcLV53-94
RBPpum3pum3
Cell_LineK562K562
Exp UID
StatusSequencingSequencing
Status_date2026-06-022026-06-02
ProjectENCORE2ENCORE2
Note
ID1480014801




Library-Prep Information
BGKcLV53-93BGKcLV53-94
Sample #5152
Sample Namepum3-BGKcLV53-93pum3-BGKcLV53-94
Sample_Name_Alias
Index Well PositionA08B08
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2026-05-262026-05-26
Lib_IDLib-260526Lib-260526
Tecan_Location
Tecan
Tecan_date
Size_bp288294
Peak_Molarity77.8079.00
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2026-05-272026-05-27
Sample_IDBGKcLV53-93BGKcLV53-94
RBPpum3pum3
Batch_IDBGKcLV53BGKcLV53
WB_result74.80072.500
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID48904891




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database