non-target      CRISPR in K562      Batch: BGKcLV53

General Information

List KD experiments controlled by BGKcLV53-1 and BGKcLV53-2


NT-BGKcLV53-1,NT-BGKcLV53-2:No KD experiments controlled by NT-BGKcLV53-1 and NT-BGKcLV53-2 are available on the database




Experiment Information (Status: Sequenced)
BGKcLV53-1BGKcLV53-2
idx00
TRCN#_or_BGC#BGC#0000000BGC#0000000
shRNA_or_gRNA_sequenceCAGTCGGGCGTCATCATGATCAGTCGGGCGTCATCATGAT
PAM
Namenon-targetnon-target
Sample_IDBGKcLV53-1BGKcLV53-2
transduction_Date2/24/262/24/26
daysD6D6
RBP_namenon-targetnon-target
qPCR_result
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc03750
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID63596360




Western Blot
Western Blot info was not avaliable




Experiment Status
BGKcLV53-1BGKcLV53-2
Sample_IDBGKcLV53-1BGKcLV53-2
Sample Name
Sample NameNT-BGKcLV53-1NT-BGKcLV53-2
RBPnon-targetnon-target
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2026-09-162026-09-16
ProjectENCORE2ENCORE2
Note
ID1478414785




Library-Prep Information
BGKcLV53-1BGKcLV53-2
Sample #3536
Sample NameNT-BGKcLV53-1NT-BGKcLV53-2
Sample_Name_Alias
Index Well PositionA06B06
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2026-05-262026-05-26
Lib_IDLib-260526Lib-260526
Tecan_Location
Tecan
Tecan_date
Size_bp290297
Peak_Molarity64.3054.40
libSampleQC_DNA_WellDNA_Library_set82/set2/A9.pngDNA_Library_set82/set2/B9.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set82/set2/A6.pngRNA_Library_set82/set2/B6.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2026-05-272026-05-27
Sample_IDBGKcLV53-1BGKcLV53-2
RBPNTNT
Batch_IDBGKcLV53BGKcLV53
WB_result0.0000.000
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID48744875




Sequencing Information
BGKcLV53-1BGKcLV53-2
Sample_IDBGKcLV53-1BGKcLV53-2
Sample NameNT-BGKcLV53-1NT-BGKcLV53-2
Pool IDPool-260528Pool-260528
LocalServer_folderset82set82
total_reads36,385,77733,338,220
total_aligned_reads31,139,93828,437,218
unique_aligned_reads28,758,15626,380,648
percent_uniqueAligned0.790370.79130
correlation_replicates0.9956200.995620
spikein_reads68,67458,262
percent_spikeins0.001890.00175
original_ReadLength151151
QC_StatusReadyToSubmitReadyToSubmit
ID35753576




Data Submission Information
DCC submission information was not avaliable




File Information
file_namefile_pathfile_formatoutput_typeassay_typelabSample IDplatformread_lengthrun_typepaired_endassemblygenome_annotationderived_fromcontrolled_byProjectNoteFILE_ID
NT-BGKcLV53-1_R1.filtered.trimmed.paired.fastq.gzENCODE_DATA/set82/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3575NovaSeq6000100paired-ended1ENCORE233682
NT-BGKcLV53-1_R2.filtered.trimmed.paired.fastq.gzENCODE_DATA/set82/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3575NovaSeq6000100paired-ended2ENCORE233740
NT-BGKcLV53-1_Aligned.sortedByCoord.out.bamENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab3575GRCh38V40NT-BGKcLV53-1_R1.filtered.trimmed.paired.fastq.gz,NT-BGKcLV53-1_R2.filtered.trimmed.paired.fastq.gzENCORE233798
NT-BGKcLV53-1_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3575GRCh38V40NT-BGKcLV53-1_Aligned.sortedByCoord.out.bamENCORE233856
NT-BGKcLV53-1_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3575GRCh38V40NT-BGKcLV53-1_Aligned.sortedByCoord.out.bamENCORE233914
NT-BGKcLV53-1_Signal.Unique.strand-.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3575GRCh38V40NT-BGKcLV53-1_Aligned.sortedByCoord.out.bamENCORE233972
NT-BGKcLV53-1_Signal.Unique.strand+.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3575GRCh38V40NT-BGKcLV53-1_Aligned.sortedByCoord.out.bamENCORE234030
NT-BGKcLV53-1_quant.sfENCODE_DATA/set82/salmon_ENCORE2_file_linkstranscript_quantificationtranscript quantificationsknockdown followed by RNA-seqGraveley Lab3575GRCh38V40NT-BGKcLV53-1_R1.filtered.trimmed.paired.fastq.gz,NT-BGKcLV53-1_R2.filtered.trimmed.paired.fastq.gzENCORE234088
NT-BGKcLV53-2_R1.filtered.trimmed.paired.fastq.gzENCODE_DATA/set82/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3576NovaSeq6000100paired-ended1ENCORE233683
NT-BGKcLV53-2_R2.filtered.trimmed.paired.fastq.gzENCODE_DATA/set82/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3576NovaSeq6000100paired-ended2ENCORE233741
NT-BGKcLV53-2_Aligned.sortedByCoord.out.bamENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab3576GRCh38V40NT-BGKcLV53-2_R1.filtered.trimmed.paired.fastq.gz,NT-BGKcLV53-2_R2.filtered.trimmed.paired.fastq.gzENCORE233799
NT-BGKcLV53-2_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3576GRCh38V40NT-BGKcLV53-2_Aligned.sortedByCoord.out.bamENCORE233857
NT-BGKcLV53-2_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3576GRCh38V40NT-BGKcLV53-2_Aligned.sortedByCoord.out.bamENCORE233915
NT-BGKcLV53-2_Signal.Unique.strand-.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3576GRCh38V40NT-BGKcLV53-2_Aligned.sortedByCoord.out.bamENCORE233973
NT-BGKcLV53-2_Signal.Unique.strand+.bwENCODE_DATA/set82/STAR_ENCORE2_trimmed_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3576GRCh38V40NT-BGKcLV53-2_Aligned.sortedByCoord.out.bamENCORE234031
NT-BGKcLV53-2_quant.sfENCODE_DATA/set82/salmon_ENCORE2_file_linkstranscript_quantificationtranscript quantificationsknockdown followed by RNA-seqGraveley Lab3576GRCh38V40NT-BGKcLV53-2_R1.filtered.trimmed.paired.fastq.gz,NT-BGKcLV53-2_R2.filtered.trimmed.paired.fastq.gzENCORE234089