ZFC3H1      CRISPR in K562      Batch: BGKcLV48

Experiment Information (Status: Sequenced)
BGKcLV48-83BGKcLV48-84
idx00
TRCN#_or_BGC#BGC#0001387BGC#0001387
shRNA_or_gRNA_sequenceGAAGGCATCCGTACAGAAGGGAAGGCATCCGTACAGAAGG
PAMCGGCGG
NameZFC3H1_83ZFC3H1_83
Sample_IDBGKcLV48-83BGKcLV48-84
transduction_Date4/15/254/15/25
daysD6D6
RBP_nameZFC3H1ZFC3H1
qPCR_result19.75.8
Ave_qPCR12.8
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc22082332
WB_result86.090.8
Ave_WB
WB_DONE_date5/28/25
MW226kd
IP1old
antibody_Cat#A301-455Alot# 001
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID62166217




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
ZFC3H1Product_ID: A301-455A
Lot_ID: 001
Source: Bethyl Labs
Target Name: ZFC3H1-human		 ZFC3H1-K562-CRISPR-A301-455A.png<br>Caption: Western blot following CRISPR against ZFC3H1 in K562 whole cell lysate using ZFC3H1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ZFC3H1. ZFC3H1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV48-83BGKcLV48-84
Sample_IDBGKcLV48-83BGKcLV48-84
Sample Name
Sample NameZFC3H1-BGKcLV48-83ZFC3H1-BGKcLV48-84
RBPZFC3H1ZFC3H1
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2025-10-022025-10-02
ProjectENCORE2ENCORE2
Note
ID1425814259




Library-Prep Information
BGKcLV48-83BGKcLV48-84
Sample #2122
Sample NameZFC3H1-BGKcLV48-83ZFC3H1-BGKcLV48-84
Sample_Name_Alias
Index Well PositionE03F03
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-182025-08-18
Lib_IDLib-250818Lib-250818
Tecan_Location
Tecan
Tecan_date
Size_bp265256
Peak_Molarity91.8084.30
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-08-192025-08-19
Sample_IDBGKcLV48-83BGKcLV48-84
RBPZFC3H1ZFC3H1
Batch_IDBGKcLV48BGKcLV48
WB_result86.00090.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47664767




Sequencing Information
BGKcLV48-83BGKcLV48-84
Sample_IDBGKcLV48-83BGKcLV48-84
Sample NameZFC3H1-BGKcLV48-83ZFC3H1-BGKcLV48-84
Pool IDPool-250822Pool-250822
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID34693470




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database