ZFC3H1      CRISPR in K562      Batch: BGKcLV48

Experiment Information (Status: LibPreping)
BGKcLV48-83BGKcLV48-84
idx00
TRCN#_or_BGC#BGC#0001387BGC#0001387
shRNA_or_gRNA_sequenceGAAGGCATCCGTACAGAAGGGAAGGCATCCGTACAGAAGG
PAMCGGCGG
NameZFC3H1_83ZFC3H1_83
Sample_IDBGKcLV48-83BGKcLV48-84
transduction_Date4/15/254/15/25
daysD6D6
RBP_nameZFC3H1ZFC3H1
qPCR_result19.75.8
Ave_qPCR12.8
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc22082332
WB_result86.090.8
Ave_WB
WB_DONE_date5/28/25
MW226kd
IP1old
antibody_Cat#A301-455Alot# 001
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID62146215




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
ZFC3H1Product_ID: A301-455A
Lot_ID: 001
Source: Bethyl Labs
Target Name: ZFC3H1-human		 ZFC3H1-K562-CRISPR-A301-455A.png<br>Caption: Western blot following CRISPR against ZFC3H1 in K562 whole cell lysate using ZFC3H1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against ZFC3H1. ZFC3H1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV48-83BGKcLV48-84
Sample_IDBGKcLV48-83BGKcLV48-84
Sample Name
Sample Name
RBPZFC3H1ZFC3H1
Cell_LineK562K562
Exp UID
StatusLibPrepingLibPreping
Status_date2025-08-062025-08-06
ProjectENCORE2ENCORE2
Note
ID1425814259




Library-Prep Information
BGKcLV48-83BGKcLV48-84
Sample #2122
Sample NameZFC3H1-BGKcLV48-83ZFC3H1-BGKcLV48-84
Sample_Name_Alias
Index Well PositionE03F03
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-182025-08-18
Lib_IDLib-250818Lib-250818
Tecan_Location
Tecan
Tecan_date
Size_bp265256
Peak_Molarity91.8084.30
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-08-192025-08-19
Sample_IDBGKcLV48-83BGKcLV48-84
RBPZFC3H1ZFC3H1
Batch_IDBGKcLV48BGKcLV48
WB_result86.00090.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusWellPositionAssignedWellPositionAssigned
ProjectENCORE2ENCORE2
ID47664767




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database