non-target      CRISPR in K562      Batch: BGKcLV48

General Information

List KD experiments controlled by BGKcLV48-1 and BGKcLV48-2


NT-BGKcLV48-1,NT-BGKcLV48-2:No KD experiments controlled by NT-BGKcLV48-1 and NT-BGKcLV48-2 are available on the database




Experiment Information (Status: Sequenced)
BGKcLV48-1BGKcLV48-2
idx00
TRCN#_or_BGC#
shRNA_or_gRNA_sequence
PAM
Namenon-targetnon-target
Sample_IDBGKcLV48-1BGKcLV48-2
transduction_Date4/15/254/15/25
daysD6D6
RBP_namenon-targetnon-target
qPCR_result
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc02097
WB_result
Ave_WB
WB_DONE_date
MW
IP
antibody_Cat#
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID61476148




Western Blot
Western Blot info was not avaliable




Experiment Status
BGKcLV48-1BGKcLV48-2
Sample_IDBGKcLV48-1BGKcLV48-2
Sample Name
Sample NameNT-BGKcLV48-1NT-BGKcLV48-2
RBPnon-targetnon-target
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2025-10-022025-10-02
ProjectENCORE2ENCORE2
Note
ID1423814239




Library-Prep Information
BGKcLV48-1BGKcLV48-2
Sample #12
Sample NameNT-BGKcLV48-1NT-BGKcLV48-2
Sample_Name_Alias
Index Well PositionA01B01
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-182025-08-18
Lib_IDLib-250818Lib-250818
Tecan_Location
Tecan
Tecan_date
Size_bp271280
Peak_Molarity78.30111.00
libSampleQC_DNA_WellDNA_Library_set79/set1/A1.pngDNA_Library_set79/set1/B1.png
RIN10.09.9
libSample_RNA_WellRNA_Library_set79/set1/A10.pngRNA_Library_set79/set1/B10.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-08-192025-08-19
Sample_IDBGKcLV48-1BGKcLV48-2
RBPNTNT
Batch_IDBGKcLV48BGKcLV48
WB_result0.0000.000
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47464747




Sequencing Information
BGKcLV48-1BGKcLV48-2
Sample_IDBGKcLV48-1BGKcLV48-2
Sample NameNT-BGKcLV48-1NT-BGKcLV48-2
Pool IDPool-250822Pool-250822
LocalServer_folderset79set79
total_reads18,383,53916,339,212
total_aligned_reads16,005,15314,205,531
unique_aligned_reads14,758,93013,160,606
percent_uniqueAligned0.802830.80546
correlation_replicates0.9979130.997913
spikein_reads62,36664,844
percent_spikeins0.003390.00397
original_ReadLength151151
QC_StatusReadyToSubmitReadyToSubmit
ID34493450




Data Submission Information
DCC submission information was not avaliable




File Information
file_namefile_pathfile_formatoutput_typeassay_typelabSample IDplatformread_lengthrun_typepaired_endassemblygenome_annotationderived_fromcontrolled_byProjectNoteFILE_ID
NT-BGKcLV48-1_S165_L002_R1_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set79/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3449NovaSeq6000100paired-ended1ENCORE232824
NT-BGKcLV48-1_S165_L002_R2_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set79/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3449NovaSeq6000100paired-ended2ENCORE232846
NT-BGKcLV48-1_Aligned.sortedByCoord.out.bamENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab3449GRCh38V40NT-BGKcLV48-1_S165_L002_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV48-1_S165_L002_R2_001.filtered.trimmed.paired.fastq.gzENCORE232868
NT-BGKcLV48-1_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3449GRCh38V40NT-BGKcLV48-1_Aligned.sortedByCoord.out.bamENCORE232890
NT-BGKcLV48-1_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3449GRCh38V40NT-BGKcLV48-1_Aligned.sortedByCoord.out.bamENCORE232912
NT-BGKcLV48-1_Signal.Unique.strand-.bwENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3449GRCh38V40NT-BGKcLV48-1_Aligned.sortedByCoord.out.bamENCORE232934
NT-BGKcLV48-1_Signal.Unique.strand+.bwENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3449GRCh38V40NT-BGKcLV48-1_Aligned.sortedByCoord.out.bamENCORE232956
NT-BGKcLV48-2_S166_L002_R1_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set79/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3450NovaSeq6000100paired-ended1ENCORE232825
NT-BGKcLV48-2_S166_L002_R2_001.filtered.trimmed.paired.fastq.gzENCODE_DATA/set79/fastq_trimmedfastqreadsknockdown followed by RNA-seqGraveley Lab3450NovaSeq6000100paired-ended2ENCORE232847
NT-BGKcLV48-2_Aligned.sortedByCoord.out.bamENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_linksbamalignmentsknockdown followed by RNA-seqGraveley Lab3450GRCh38V40NT-BGKcLV48-2_S166_L002_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV48-2_S166_L002_R2_001.filtered.trimmed.paired.fastq.gzENCORE232869
NT-BGKcLV48-2_Signal.UniqueMultiple.strand-.bwENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_linksbigWig-allminus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3450GRCh38V40NT-BGKcLV48-2_Aligned.sortedByCoord.out.bamENCORE232891
NT-BGKcLV48-2_Signal.UniqueMultiple.strand+.bwENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_linksbigWig+allplus strand signal of all readsknockdown followed by RNA-seqGraveley Lab3450GRCh38V40NT-BGKcLV48-2_Aligned.sortedByCoord.out.bamENCORE232913
NT-BGKcLV48-2_Signal.Unique.strand-.bwENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_linksbigWig-uniqueminus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3450GRCh38V40NT-BGKcLV48-2_Aligned.sortedByCoord.out.bamENCORE232935
NT-BGKcLV48-2_Signal.Unique.strand+.bwENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_linksbigWig+uniqueplus strand signal of unique readsknockdown followed by RNA-seqGraveley Lab3450GRCh38V40NT-BGKcLV48-2_Aligned.sortedByCoord.out.bamENCORE232957