RBM33      CRISPR in K562      Batch: BGKcLV48

Experiment Information (Status: Sequenced)
BGKcLV48-51BGKcLV48-51BGKcLV48-52BGKcLV48-52
idx0000
TRCN#_or_BGC#BGC#0001362BGC#0001362BGC#0001362BGC#0001362
shRNA_or_gRNA_sequenceGGGCCCCGCGAGTGCCATGGGGGCCCCGCGAGTGCCATGGGGGCCCCGCGAGTGCCATGGGGGCCCCGCGAGTGCCATGG
PAMCGGCGGCGGCGG
NameRBM33_75RBM33_75RBM33_75RBM33_75
Sample_IDBGKcLV48-51BGKcLV48-51BGKcLV48-52BGKcLV48-52
transduction_Date4/15/254/15/254/15/254/15/25
daysD6D6D6D6
RBP_nameRBM33RBM33RBM33RBM33
qPCR_result32.632.614.714.7
Ave_qPCR23.723.7
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc2096209621112111
WB_result86.962.685.857.5
Ave_WB
WB_DONE_date5/8/255/28/25
MW130KD130KD
IP
antibody_Cat#A303-926AA303-927ALOT #1LOT #1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM0000
Rep2_TPM0000
ActionReadyReady
Library_start_date
repeat_library
Note
ID6182618461836185




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RBM33Product_ID: A303-926A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RBM33-human		 Bethyl_A303-926A_1_RBM33.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RBM33 specific antibody, A303-926A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RBM33-specific antibody, A303-926A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RBM33-K562-CRISPR-A303-926A.png<br>Caption: Western blot following CRISPR against RBM33 in K562 whole cell lysate using RBM33 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM33. RBM33 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
RBM33Product_ID: A303-927A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RBM33-human		 Bethyl_A303-927A_1_RBM33.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RBM33 specific antibody, A303-927A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RBM33-specific antibody, A303-927A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RBM33-K562-CRISPR-A303-927A.png<br>Caption: Western blot following CRISPR against RBM33 in K562 whole cell lysate using RBM33 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM33. RBM33 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV48-51BGKcLV48-51BGKcLV48-52BGKcLV48-52
Sample_IDBGKcLV48-51BGKcLV48-51BGKcLV48-52BGKcLV48-52
Sample Name
Sample NameRBM33-BGKcLV48-51RBM33-BGKcLV48-52
RBPRBM33RBM33RBM33RBM33
Cell_LineK562K562K562K562
Exp UID
StatusSequencedNotSatisfiedSequencedNotSatisfied
Status_date2025-10-022025-08-062025-10-022025-08-06
ProjectENCORE2ENCORE2ENCORE2ENCORE2
Note
ID14248143001424914301




Library-Prep Information
BGKcLV48-51BGKcLV48-52
Sample #1112
Sample NameRBM33-BGKcLV48-51RBM33-BGKcLV48-52
Sample_Name_Alias
Index Well PositionC02D02
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-182025-08-18
Lib_IDLib-250818Lib-250818
Tecan_Location
Tecan
Tecan_date
Size_bp279268
Peak_Molarity98.00114.00
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-08-192025-08-19
Sample_IDBGKcLV48-51BGKcLV48-52
RBPRBM33RBM33
Batch_IDBGKcLV48BGKcLV48
WB_result86.90085.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47564757




Sequencing Information
BGKcLV48-51BGKcLV48-52
Sample_IDBGKcLV48-51BGKcLV48-52
Sample NameRBM33-BGKcLV48-51RBM33-BGKcLV48-52
Pool IDPool-250822Pool-250822
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID34593460




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database