RBM33      CRISPR in K562      Batch: BGKcLV48

Experiment Information (Status: Sequencing)
BGKcLV48-51BGKcLV48-51BGKcLV48-52BGKcLV48-52
idx0000
TRCN#_or_BGC#BGC#0001362BGC#0001362BGC#0001362BGC#0001362
shRNA_or_gRNA_sequenceGGGCCCCGCGAGTGCCATGGGGGCCCCGCGAGTGCCATGGGGGCCCCGCGAGTGCCATGGGGGCCCCGCGAGTGCCATGG
PAMCGGCGGCGGCGG
NameRBM33_75RBM33_75RBM33_75RBM33_75
Sample_IDBGKcLV48-51BGKcLV48-51BGKcLV48-52BGKcLV48-52
transduction_Date4/15/254/15/254/15/254/15/25
daysD6D6D6D6
RBP_nameRBM33RBM33RBM33RBM33
qPCR_result32.632.614.714.7
Ave_qPCR23.723.7
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc2096209621112111
WB_result86.962.685.857.5
Ave_WB
WB_DONE_date5/8/255/28/25
MW130KD130KD
IP
antibody_Cat#A303-926AA303-927ALOT #1LOT #1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM0000
Rep2_TPM0000
ActionReadyReady
Library_start_date
repeat_library
Note
ID6180618261816183




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RBM33Product_ID: A303-926A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RBM33-human		 Bethyl_A303-926A_1_RBM33.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RBM33 specific antibody, A303-926A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RBM33-specific antibody, A303-926A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RBM33-K562-CRISPR-A303-926A.png<br>Caption: Western blot following CRISPR against RBM33 in K562 whole cell lysate using RBM33 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM33. RBM33 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
RBM33Product_ID: A303-927A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RBM33-human		 Bethyl_A303-927A_1_RBM33.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RBM33 specific antibody, A303-927A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RBM33-specific antibody, A303-927A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RBM33-K562-CRISPR-A303-927A.png<br>Caption: Western blot following CRISPR against RBM33 in K562 whole cell lysate using RBM33 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBM33. RBM33 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV48-51BGKcLV48-51BGKcLV48-52BGKcLV48-52
Sample_IDBGKcLV48-51BGKcLV48-51BGKcLV48-52BGKcLV48-52
Sample Name
Sample NameRBM33-BGKcLV48-51RBM33-BGKcLV48-52
RBPRBM33RBM33RBM33RBM33
Cell_LineK562K562K562K562
Exp UID
StatusSequencingNotSatisfiedSequencingNotSatisfied
Status_date2025-09-102025-08-062025-09-102025-08-06
ProjectENCORE2ENCORE2ENCORE2ENCORE2
Note
ID14248143001424914301




Library-Prep Information
BGKcLV48-51BGKcLV48-52
Sample #1112
Sample NameRBM33-BGKcLV48-51RBM33-BGKcLV48-52
Sample_Name_Alias
Index Well PositionC02D02
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-182025-08-18
Lib_IDLib-250818Lib-250818
Tecan_Location
Tecan
Tecan_date
Size_bp279268
Peak_Molarity98.00114.00
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-08-192025-08-19
Sample_IDBGKcLV48-51BGKcLV48-52
RBPRBM33RBM33
Batch_IDBGKcLV48BGKcLV48
WB_result86.90085.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47564757




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database