DARS RNA-Seq in K562

Experiment Information (Status: Sequenced)

	BGKcLV48-17	BGKcLV48-18
idx	0	0
TRCN#_or_BGC#	BGC#0001326	BGC#0001326
shRNA_or_gRNA_sequence	CATTTGCTTATATAGCTGTG	CATTTGCTTATATAGCTGTG
PAM	GGG	GGG
Name	DARS1_73	DARS1_73
Sample_ID	BGKcLV48-17	BGKcLV48-18
transduction_Date	4/15/25	4/15/25
days	D6	D6
RBP_name	DARS	DARS
qPCR_result	71.2	62.5
Ave_qPCR
RT-qPCR_primer-F	tgaatcagaaaattggaagctg
RT-qPCR_primer-R	ggggttcagccaaactgat
protein_conc	1808	2132
WB_result	60.1	56.7453
Ave_WB
WB_DONE_date	8/1/25
MW	57KD
IP	Rabbit / I
antibody_Cat#	A304-799A	LOT #1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM	0	0
Rep2_TPM	0	0
Action	Ready	Ready
Library_start_date
repeat_library
Note
ID	6163	6164

Western Blot

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
DARS	Product_ID: A304-799A Lot_ID: 1 Source: Bethyl Labs Target Name: DARS-human

Experiment Status

	BGKcLV48-17	BGKcLV48-18
Sample_ID	BGKcLV48-17	BGKcLV48-18
Sample Name
Sample Name	DARS-BGKcLV48-17	DARS-BGKcLV48-18
RBP	DARS	DARS
Cell_Line	K562	K562
Exp UID
Status	Sequenced	Sequenced
Status_date	2025-10-02	2025-10-02
Project	ENCORE2	ENCORE2
Note
ID	14240	14241

Library-Prep
Sequencing

Library-Prep Information

	BGKcLV48-17	BGKcLV48-18
Sample #	3	4
Sample Name	DARS-BGKcLV48-17	DARS-BGKcLV48-18
Sample_Name_Alias
Index Well Position	C01	D01
Index_table	IDT_UniqueDualIndex_96_V2	IDT_UniqueDualIndex_96_V2
LibPrep_date	2025-08-18	2025-08-18
Lib_ID	Lib-250818	Lib-250818
Tecan_Location
Tecan
Tecan_date
Size_bp	272	276
Peak_Molarity	86.60	101.00
libSampleQC_DNA_Well	DNA_Library_set79/set1/C1.png	DNA_Library_set79/set1/D1.png
RIN	10.0	9.9
libSample_RNA_Well	RNA_Library_set79/set1/C10.png	RNA_Library_set79/set1/D10.png
SampleQC_method	TapeStation_2022	TapeStation_2022
SampleQC_date	2025-08-19	2025-08-19
Sample_ID	BGKcLV48-17	BGKcLV48-18
RBP	DARS	DARS
Batch_ID	BGKcLV48	BGKcLV48
WB_result	60.100	56.745
Library Description	TruSeq mRNA	TruSeq mRNA
Repeat_Library_Suffix
Lib_Status	SendToSequence	SendToSequence
Project	ENCORE2	ENCORE2
ID	4748	4749

Sequencing Information

	BGKcLV48-17	BGKcLV48-18
Sample_ID	BGKcLV48-17	BGKcLV48-18
Sample Name	DARS-BGKcLV48-17	DARS-BGKcLV48-18
Pool ID	Pool-250822	Pool-250822
LocalServer_folder	set79	set79
total_reads	15,960,326	18,000,880
total_aligned_reads	13,480,016	15,799,413
unique_aligned_reads	12,452,421	14,606,136
percent_uniqueAligned	0.78021	0.81141
correlation_replicates	0.993531	0.993531
spikein_reads	39,690	84,524
percent_spikeins	0.00249	0.00470
original_ReadLength	151	151
QC_Status	ReadyToSubmit	ReadyToSubmit
ID	3451	3452

Data Submission Information DCC submission information was not avaliable

File Information

file_name	file_path	file_format	output_type	assay_type	lab	Sample ID	platform	read_length	run_type	paired_end	assembly	genome_annotation	derived_from	controlled_by	Project	FILE_ID
DARS-BGKcLV48-17_S167_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set79/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3451	NovaSeq6000	100	paired-ended	1				NT-BGKcLV48-1_S165_L002_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV48-2_S166_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	32820
DARS-BGKcLV48-17_S167_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set79/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3451	NovaSeq6000	100	paired-ended	2				NT-BGKcLV48-1_S165_L002_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV48-2_S166_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	32842
DARS-BGKcLV48-17_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3451					GRCh38	V40	DARS-BGKcLV48-17_S167_L002_R1_001.filtered.trimmed.paired.fastq.gz,DARS-BGKcLV48-17_S167_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	32864
DARS-BGKcLV48-17_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3451					GRCh38	V40	DARS-BGKcLV48-17_Aligned.sortedByCoord.out.bam		ENCORE2	32886
DARS-BGKcLV48-17_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3451					GRCh38	V40	DARS-BGKcLV48-17_Aligned.sortedByCoord.out.bam		ENCORE2	32908
DARS-BGKcLV48-17_Signal.Unique.strand-.bw	ENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3451					GRCh38	V40	DARS-BGKcLV48-17_Aligned.sortedByCoord.out.bam		ENCORE2	32930
DARS-BGKcLV48-17_Signal.Unique.strand+.bw	ENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3451					GRCh38	V40	DARS-BGKcLV48-17_Aligned.sortedByCoord.out.bam		ENCORE2	32952
DARS-BGKcLV48-18_S168_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set79/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3452	NovaSeq6000	100	paired-ended	1				NT-BGKcLV48-1_S165_L002_R1_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV48-2_S166_L002_R1_001.filtered.trimmed.paired.fastq.gz	ENCORE2	32821
DARS-BGKcLV48-18_S168_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCODE_DATA/set79/fastq_trimmed	fastq	reads	knockdown followed by RNA-seq	Graveley Lab	3452	NovaSeq6000	100	paired-ended	2				NT-BGKcLV48-1_S165_L002_R2_001.filtered.trimmed.paired.fastq.gz,NT-BGKcLV48-2_S166_L002_R2_001.filtered.trimmed.paired.fastq.gz	ENCORE2	32843
DARS-BGKcLV48-18_Aligned.sortedByCoord.out.bam	ENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_links	bam	alignments	knockdown followed by RNA-seq	Graveley Lab	3452					GRCh38	V40	DARS-BGKcLV48-18_S168_L002_R1_001.filtered.trimmed.paired.fastq.gz,DARS-BGKcLV48-18_S168_L002_R2_001.filtered.trimmed.paired.fastq.gz		ENCORE2	32865
DARS-BGKcLV48-18_Signal.UniqueMultiple.strand-.bw	ENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_links	bigWig-all	minus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3452					GRCh38	V40	DARS-BGKcLV48-18_Aligned.sortedByCoord.out.bam		ENCORE2	32887
DARS-BGKcLV48-18_Signal.UniqueMultiple.strand+.bw	ENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_links	bigWig+all	plus strand signal of all reads	knockdown followed by RNA-seq	Graveley Lab	3452					GRCh38	V40	DARS-BGKcLV48-18_Aligned.sortedByCoord.out.bam		ENCORE2	32909
DARS-BGKcLV48-18_Signal.Unique.strand-.bw	ENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_links	bigWig-unique	minus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3452					GRCh38	V40	DARS-BGKcLV48-18_Aligned.sortedByCoord.out.bam		ENCORE2	32931
DARS-BGKcLV48-18_Signal.Unique.strand+.bw	ENCODE_DATA/set79/STAR_ENCORE2_trimmed_file_links	bigWig+unique	plus strand signal of unique reads	knockdown followed by RNA-seq	Graveley Lab	3452					GRCh38	V40	DARS-BGKcLV48-18_Aligned.sortedByCoord.out.bam		ENCORE2	32953