DARS      CRISPR in K562      Batch: BGKcLV48

Experiment Information (Status: Sequencing)
BGKcLV48-17BGKcLV48-18
idx00
TRCN#_or_BGC#BGC#0001326BGC#0001326
shRNA_or_gRNA_sequenceCATTTGCTTATATAGCTGTGCATTTGCTTATATAGCTGTG
PAMGGGGGG
NameDARS1_73DARS1_73
Sample_IDBGKcLV48-17BGKcLV48-18
transduction_Date4/15/254/15/25
daysD6D6
RBP_nameDARSDARS
qPCR_result71.262.5
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc18082132
WB_result60.156.7453
Ave_WB
WB_DONE_date8/1/25
MW57KD
IPRabbit / I
antibody_Cat#A304-799ALOT #1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID61466147




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
DARSProduct_ID: A304-799A
Lot_ID: 1
Source: Bethyl Labs
Target Name: DARS-human		 DARS1-HEPG2-CRISPR-A304-799A.png<br>Caption: Western blot following CRISPR against DARS in HepG2 whole cell lysate using DARS specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DARS. DARS protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 Bethyl_A304-799A_1_DARS.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the DARS specific antibody, A304-799A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DARS-specific antibody, A304-799A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 DARS-K562-CRISPR-A304-799A.png<br>Caption: Western blot following CRISPR against DARS in K562 whole cell lysate using DARS specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DARS. DARS protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV48-17BGKcLV48-18
Sample_IDBGKcLV48-17BGKcLV48-18
Sample Name
Sample NameDARS-BGKcLV48-17DARS-BGKcLV48-18
RBPDARSDARS
Cell_LineK562K562
Exp UID
StatusSequencingSequencing
Status_date2025-09-102025-09-10
ProjectENCORE2ENCORE2
Note
ID1424014241




Library-Prep Information
BGKcLV48-17BGKcLV48-18
Sample #34
Sample NameDARS-BGKcLV48-17DARS-BGKcLV48-18
Sample_Name_Alias
Index Well PositionC01D01
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-182025-08-18
Lib_IDLib-250818Lib-250818
Tecan_Location
Tecan
Tecan_date
Size_bp272276
Peak_Molarity86.60101.00
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-08-192025-08-19
Sample_IDBGKcLV48-17BGKcLV48-18
RBPDARSDARS
Batch_IDBGKcLV48BGKcLV48
WB_result60.10056.745
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47484749




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database