DARS      CRISPR in K562      Batch: BGKcLV48

Experiment Information (Status: Sequenced)
BGKcLV48-17BGKcLV48-18
idx00
TRCN#_or_BGC#BGC#0001326BGC#0001326
shRNA_or_gRNA_sequenceCATTTGCTTATATAGCTGTGCATTTGCTTATATAGCTGTG
PAMGGGGGG
NameDARS1_73DARS1_73
Sample_IDBGKcLV48-17BGKcLV48-18
transduction_Date4/15/254/15/25
daysD6D6
RBP_nameDARSDARS
qPCR_result71.262.5
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc18082132
WB_result60.156.7453
Ave_WB
WB_DONE_date8/1/25
MW57KD
IPRabbit / I
antibody_Cat#A304-799ALOT #1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID61486149




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
DARSProduct_ID: A304-799A
Lot_ID: 1
Source: Bethyl Labs
Target Name: DARS-human		 DARS1-HEPG2-CRISPR-A304-799A.png<br>Caption: Western blot following CRISPR against DARS in HepG2 whole cell lysate using DARS specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DARS. DARS protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 Bethyl_A304-799A_1_DARS.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the DARS specific antibody, A304-799A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DARS-specific antibody, A304-799A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 DARS-K562-CRISPR-A304-799A.png<br>Caption: Western blot following CRISPR against DARS in K562 whole cell lysate using DARS specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DARS. DARS protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV48-17BGKcLV48-18
Sample_IDBGKcLV48-17BGKcLV48-18
Sample Name
Sample NameDARS-BGKcLV48-17DARS-BGKcLV48-18
RBPDARSDARS
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2025-10-022025-10-02
ProjectENCORE2ENCORE2
Note
ID1424014241




Library-Prep Information
BGKcLV48-17BGKcLV48-18
Sample #34
Sample NameDARS-BGKcLV48-17DARS-BGKcLV48-18
Sample_Name_Alias
Index Well PositionC01D01
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-182025-08-18
Lib_IDLib-250818Lib-250818
Tecan_Location
Tecan
Tecan_date
Size_bp272276
Peak_Molarity86.60101.00
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2025-08-192025-08-19
Sample_IDBGKcLV48-17BGKcLV48-18
RBPDARSDARS
Batch_IDBGKcLV48BGKcLV48
WB_result60.10056.745
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID47484749




Sequencing Information
BGKcLV48-17BGKcLV48-18
Sample_IDBGKcLV48-17BGKcLV48-18
Sample NameDARS-BGKcLV48-17DARS-BGKcLV48-18
Pool IDPool-250822Pool-250822
LocalServer_folder
total_reads00
total_aligned_reads00
unique_aligned_reads00
percent_uniqueAligned
correlation_replicates
spikein_reads00
percent_spikeins
original_ReadLength
QC_StatusReadyToQCReadyToQC
ID34513452




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database