DARS antibody: A304-799A


Antibody Info
Product_IDA304-799A
Lot_ID1
Host_organismrabbit
SourceBethyl Labs
Antibody_DCC_IDENCAB187BNV
Target Info
GeneNameDARS
Phosphate
Target_nameDARS-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 DARS1-HEPG2-CRISPR-A304-799A.png<br>Caption: Western blot following CRISPR against DARS in HepG2 whole cell lysate using DARS specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DARS. DARS protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against DARS in HepG2 whole cell lysate using DARS specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DARS. DARS protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.

Releated Sample: BGHcLV29-57

Status: Released

Lab: Graveley Lab

K562 Bethyl_A304-799A_1_DARS.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the DARS specific antibody, A304-799A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DARS-specific antibody, A304-799A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Caption: IP-WB analysis of K562 whole cell lysate using the DARS specific antibody, A304-799A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DARS-specific antibody, A304-799A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Method: immunoprecipitation

Status: Released

Lab: Yeo Lab