MARS      CRISPR in K562      Control: NT-BGKcLV46-1,NT-BGKcLV46-2

General Information
RBPMARS
Cell_LineK562
MethodCRISPR
Exp_NameMARS-BGKcLV46-K562
ENCODE_series_ID
Batch_IDBGKcLV46
Pool IDPool-250312
Local_Set_Nameset77
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1MARS-BGKcLV46-45
Rep2MARS-BGKcLV46-46
CN1NT-BGKcLV46-1
CN2NT-BGKcLV46-2
Rep1_qPCR60.3
Rep2_qPCR57.4
Rep1_WB87.7
Rep2_WB87.5
Antibody Cat#A303-960A
Antibody Lot#lot# 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1643




Experiment Information (Status: NotSatisfied)
BGKcLV46-47BGKcLV46-48
idx00
TRCN#_or_BGC#BGC#0001031BGC#0001031
shRNA_or_gRNA_sequenceCCCGGGTTGCTTGCCGGTGCCCCGGGTTGCTTGCCGGTGC
PAMTGGTGG
NameMARS_82BMARS_82B
Sample_IDBGKcLV46-47BGKcLV46-48
transduction_Date12/20/2412/20/24
daysD6D6
RBP_nameMARSMARS
qPCR_result
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc39394096
WB_result78.992.2
Ave_WB
WB_DONE_date1/6/25
MW
IP
antibody_Cat#A303-960Alot# 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
Action
Library_start_date
repeat_library
Note
ID60416042




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
MARSProduct_ID: A303-960A
Lot_ID: 1
Source: Bethyl Labs
Target Name: MARS-human		 MARS-K562-CRISPR-A303-960A.png<br>Caption: Western blot following CRISPR against MARS in K562 whole cell lysate using MARS specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against MARS. MARS protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV46-47BGKcLV46-48
Sample_IDBGKcLV46-47BGKcLV46-48
Sample Name
Sample Name
RBPMARSMARS
Cell_LineK562K562
Exp UID
StatusNotSatisfiedNotSatisfied
Status_date2025-02-102025-02-10
ProjectENCORE2ENCORE2
Note
ID1410014101




Library-Prep Information
Library info was not avaliable




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database