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GFPT1 RNA-Seq in K562



GFPT1      CRISPR in K562      Control:

General Information
RBPGFPT1
Cell_LineK562
MethodCRISPR
Exp_NameGFPT1-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1GFPT1-BGKcLV42-37
Rep2GFPT1-BGKcLV42-38
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR68.8
Rep2_qPCR60.8
Rep1_WB84.6
Rep2_WB85.7
Antibody Cat#5322S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1541




Experiment Information (Status: Sequenced)
BGKcLV42-37BGKcLV42-38
idx00
TRCN#_or_BGC#BGC#0001139BGC#0001139
shRNA_or_gRNA_sequenceATCCTGGAGACCCTAATCAAATCCTGGAGACCCTAATCAA
PAMAGGAGG
NameGFPT1_81GFPT1_81
Sample_IDBGKcLV42-37BGKcLV42-38
transduction_Date4/23/244/23/24
daysD6D6
RBP_nameGFPT1GFPT1
qPCR_result68.860.8
Ave_qPCR64.8
RT-qPCR_primer-Fgcctgcaaaatccagcttat
RT-qPCR_primer-Rcccaacgggtatgagctatt
protein_conc27342764
WB_result84.685.7
Ave_WB85.2
WB_DONE_date5/6/24
MW80kd
IP
antibody_Cat#5322Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID57975798




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
GFPT1Product_ID: 5322S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: GFPT1-human		 GFPT1-HEPG2-CRISPR-5322S.png<br>Caption: Western blot following CRISPR against GFPT1 in HepG2 whole cell lysate using GFPT1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GFPT1. GFPT1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_5322S_1_GFPT1.png<br>Caption: IP-WB analysis of 5322S whole cell lysate using the GFPT1 specific antibody, 5322S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the GFPT1-specific antibody, 5322S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 GFPT1-K562-CRISPR-5322S.png<br>Caption: Western blot following CRISPR against GFPT1 in K562 whole cell lysate using GFPT1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against GFPT1. GFPT1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-37BGKcLV42-38
Sample_IDBGKcLV42-37BGKcLV42-38
Sample Name
Sample NameGFPT1-BGKcLV42-37GFPT1-BGKcLV42-38
RBPGFPT1GFPT1
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1307613077




Library-Prep Information
BGKcLV42-37BGKcLV42-38
Sample #3334
Sample NameGFPT1-BGKcLV42-37GFPT1-BGKcLV42-38
Sample_Name_Alias
Index Well PositionA05B05
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp316297
Peak_Molarity113.00127.00
libSampleQC_DNA_WellDNA_Library_set73/set1/A5.pngDNA_Library_set73/set1/B5.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set73/set1/A5.pngRNA_Library_set73/set1/B5.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-37BGKcLV42-38
RBPGFPT1GFPT1
Batch_IDBGKcLV42BGKcLV42
WB_result84.60085.700
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43024303




Sequencing Information
BGKcLV42-37BGKcLV42-38
Sample_IDBGKcLV42-37BGKcLV42-38
Sample NameGFPT1-BGKcLV42-37GFPT1-BGKcLV42-38
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads108,607,61431,554,848
total_aligned_reads97,189,81628,961,347
unique_aligned_reads90,085,68726,748,454
percent_uniqueAligned0.829460.84768
correlation_replicates0.9961370.996137
spikein_reads9,516938
percent_spikeins0.000090.00003
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID31693170




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database